Results 151 to 160 of about 44,886 (288)

PWO proteins are associated with PRC2 since their emergence in vascular plants

New Phytologist, EarlyView.
Summary The Polycomb repressive complex 2 (PRC2), a conserved histone methyltransferase complex, plays a central role in transcriptional silencing across eukaryotes. Here, we investigate the evolution of PWWP‐DOMAIN INTERACTORS OF POLYCOMBS (PWOs), which interact with PRC2, and examine the conservation of the PWOs‐PRC2 interaction across plant ...
Ahamed Khan, Saqlain Haider, Abdoallah Sharaf, Alžbeta Kusová, Jan Skalák, Claire Jourdain, Martin Rennie, Petra Procházková Schrumpfová, Jan Hejátko, Daniel Schubert, Sara Farrona, Iva Mozgová   +11 more
wiley   +1 more source

Harnessing Bulk‐Segregant Mapping to Identify Trait‐Associated Genes in the Allopolyploid Model Plant Nicotiana benthamiana

Plant Biotechnology Journal, EarlyView.
ABSTRACT Forward genetics has been instrumental in identifying genes underlying desirable traits, yet its application to polyploid plants, many of which are key agricultural crops, remains challenging due to their genomic complexity. Therefore, we developed BenthMap, a bulk segregant analysis platform for high‐throughput trait mapping and gene ...
Zuba Ahmed, Jiyuan An, Satomi Hayashi, Julia Bally, Chris Winefield, Peter M. Waterhouse   +5 more
wiley   +1 more source

Rooting Conifer Genetic Research: An Accessible and Efficient Transformation System

Plant Biotechnology Journal, EarlyView.
ABSTRACT Conifers serve as the cornerstone of global forest ecosystems, yet their genetic transformation faces notorious challenges. To overcome the intrinsic resistance of conifer adventitious roots to Agrobacterium/Rhizobium‐mediated transformation, we systematically and iteratively engineered the binary vector by introducing chimeric Ri plasmid ...
Jing‐Jing Li, Yi‐Mei Zhao, Jia‐Fan Liu, Chen‐He Li, Hui Zhang, Yi‐Tong Song, Quan Zuo, Pei‐Yi Wang, Kai Gao, Dong Meng, Shi‐Hui Niu   +10 more
wiley   +1 more source

Knocking Out Two Polyphenol Oxidase Genes Significantly Improves Recombinant Protein Purification in Nicotiana benthamiana

Plant Biotechnology Journal, EarlyView.
ABSTRACT Efficient purification remains a key technical challenge affecting the recovery efficiency of recombinant proteins in plant‐based systems. Complex metabolites, particularly polyphenols, often cause recombinant protein aggregation during purification.
Hai‐Ping Diao, Hui‐Xin Meng, Xue‐Jiao Xu, Zeng‐Lin Zhang, Jian‐Feng Zhang, Yong‐Feng Guo, Inhwan Hwang, Shi‐Jian Song   +7 more
wiley   +1 more source

Genska transformacija rastline Nicotiana benthamiana Domin

, 2017
The thesis has confirmed transmission and steady genetic transformation Cas9, Hpt II and ZsGreen into the plant Nicotiana benthamiana, which is gaining on the importance as a model organism. The gene transformation was carried out with the aid of a bacterium Agrobacterium tumefaciens which helped us to co-cultivate the leafy parts and which enabled ...
openaire   +1 more source

CpERF‐WRI1 Manipulates Ethylene Sensing by Regulating the Expression of CpERS1 and Fruit Ripening in Papaya

Plant Biotechnology Journal, EarlyView.
ABSTRACT Ethylene plays a crucial role in fruit ripening and is perceived by specialised receptor proteins embedded in the endoplasmic reticulum (ER) membrane. As an ethylene antagonist, 1‐methylcyclopropene (1‐MCP) binds to these receptors and delays papaya ripening, but improper use can cause ripening disorders.
Ziling Wu, Jiahui Cai, Faiz Ur Rahman, Wang Zheng, Qiunan Zhu, Weixin Chen, Xueping Li, Xiaoyang Zhu   +7 more
wiley   +1 more source

Host‐Induced Silencing of Rhizoctonia Solani 5‐Enolpyruvylshikimate‐3‐Phosphate Synthase Impairs Its Virulence in Rice

Plant Biotechnology Journal, EarlyView.
ABSTRACT Sheath blight disease of rice caused by the fungal pathogen R. solani AG1‐IA remains a big threat to rice production worldwide. A limited genetic variation in rice for tolerance to this pathogen and little success in understanding how it defeats host defence are major reasons behind it.
Vinod Kumar, Apoorva Prasad, Akshita Goel, Vijay Kumar Sharma, Jagjeet Singh Lore, Arun Kumar   +5 more
wiley   +1 more source

Ubiquitination‐Mediated Degradation of SlVPS29 by the SlHSFA7‐SlRNF185 Module Enhances Tomato Pollen Thermotolerance

Plant Biotechnology Journal, EarlyView.
ABSTRACT High temperature has posed significant challenges to global agriculture, markedly leading to reduced fertility and yield losses in tomato (Solanum lycopersicum). Therefore, thermotolerance‐conferring genes and loci are needed to further improve cultivated tomatoes.
Xiaolin Geng, Qinqin Yang, Hongwei Li, Xunwen Peng, Wenbo Zhang, Huihui Wang, Yushan Li, Fang Li, Quanquan Chen, Tao Lin   +9 more
wiley   +1 more source

Disabling posttranscriptional regulation of AGO2 results in enhanced viral resistance

New Phytologist, EarlyView.
Márta Ludman, Károly Fátyol
wiley   +1 more source

The Structure of Carboxyl Methyltransferase Provides Insights Into the Substrate Specificity and Divergent Evolution of Iridoid

Plant Biotechnology Journal, EarlyView.
ABSTRACT Iridoids constitute a prominent class of plant‐specialised metabolites, with carbocyclic iridoids (e.g., geniposide) and secoiridoids (e.g., loganin) diverging early in their biosynthetic pathways. This divergence is marked by substrate‐specific carboxyl methyltransferases—GjGAMT and CrLAMT—that catalyse the decisive methylation step in ...
Li Li, Zhiteng Luo, Zhaoyin He, Jie Tang, Wuke Wei, Ruoting Zhan, Huihao Zhou, Dongming Ma   +7 more
wiley   +1 more source