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Nicotiana benthamiana

2019
Published as part of Andersen, Trine Bundgaard, Rasmussen, Silas Anselm, Christensen, Søren Brøgger & Simonsen, Henrik Toft, 2019, Biosynthesis of tovarol and other sesquiterpenoids in Thapsia laciniata Rouy, pp. 168-174 in Phytochemistry 157 on page 169, DOI: 10.1016/j.phytochem.2018.10.027, http://zenodo.org/record ...
Andersen, Trine Bundgaard   +3 more
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Nicotiana (Nicotiana tobaccum, Nicotiana benthamiana).

Methods in molecular biology (Clifton, N.J.), 2006
Agrobacterium-mediated transformation of Nicotiana species, namely, Nicotiana tobaccum and Nicotiana benthamiana, using leaf disks as the target explant has provided the plant community with a valuable tool for rapid evaluation of transgenes in higher plants.
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Reconstitution of Metabolic Pathway in Nicotiana benthamiana

2021
Reconstitution of metabolite biosynthesis pathway plays a pivotal role in functional characterization of biosynthesis enzymes and metabolite bioengineering. Traditionally, metabolic pathways are reconstituted in bacteria or yeast due to their ease for genetic manipulation and transformation.
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Transient Transactivation Studies in Nicotiana benthamiana Leaves

2018
Transitory gene expression systems in Nicotiana benthamiana leaves, in combination with the use of gene silencing suppressors as the p19 or HC-pro proteins that allow for elevated levels of gene expression, have proven to be a highly versatile tool to analyze transcriptional function of DNA binding factors in the activated or repressed expression of ...
Pilar, Lasierra, Salomé, Prat
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Stable Plastid Transformation in Nicotiana benthamiana

Journal of Plant Biology, 2009
Plastids from Nicotiana benthamiana were transformed with the vector for dicistronic expression of two genes—aminoglycoside 3'-adenyltransferase (aadA) and green fluorescent protein (gfp)—in the plastids of Nicotiana tabacum. Transplastomic shoots exhibited green fluorescence under UV light.
Seyed Javad Davarpanah   +6 more
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Targeted Insertion in Nicotiana benthamiana Genomes via Protoplast Regeneration

2023
Insertion of a specific sequence in a targeted region for precise editing is still a major challenge in plants. Current protocols rely on inefficient homology-directed repair or non-homologous end-joining with modified double-stranded oligodeoxyribonucleotides (dsODNs) as donors.
Fu-Hui, Wu   +2 more
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Engineering montbretin A biosynthesis in Nicotiana benthamiana

2021
The plant kingdom has always been a useful source of therapeutic molecules for humans. Montbretin A (MbA) is a complex acylated flavonol glycoside found in the underground storage organs, called corms, of the ornamental plant montbretia (Crocosmia x crocosmiiflora). MbA was discovered as a highly efficient and specific inhibitor of the human pancreatic
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Characterization of in vivo functions of Nicotiana benthamiana RabE1

Planta, 2012
We characterized the gene expression, subcellular localization, and in vivo functions of a Nicotiana benthamiana small GTPase belonging to the RabE family, designated NbRabE1. The NbRabE1 promoter drove strong β-glucuronidase reporter expression in young tissues containing actively dividing cells and in stomata guard cells.
Chang Sook, Ahn   +2 more
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?????????????????? ?????????????????????? ???????????? nicotiana benthamiana, ?? ???????? ?????????????????? ?????????????????? ?????????? ????????????-4-???????????????????????? ????????????

2021
Aims. The use of plants as a recombinant peptides expression system is a valuable alternative for the production of collagen for various medical and scientific purposes. The aim of this work was to obtained transgenic plants of Nicotiana benthamiana with two human genes encoding subunits of prolyl-4-hydroxylase (P4H), responsible for key ...
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?????????????????? ?????????????????????????????? ???????????? Nicotiana benthamiana ???? ???????????? ???????????????????????? (Mycobacterium tubertulosis)

2021
Set of vectors has been created for plastome transformation of plants of Nicotiana genera. These constructs possess esxA and fbpB genes combined with selective aadA gene which are expressed polycistronicly under control of psbA gene. Over 30 antibiotic resistant lines have been selected in the course of N. benthamiana plastome transformation and a part
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