Results 301 to 310 of about 20,026 (345)
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Properties of a nitrate reductase of Chlorella
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1972Abstract 1. An NADH-nitrate oxidoreductase (EC 1.6.6.1) of Chlorella has the unusual property of existing in cell-free extracts mainly in the form of an inactive precursor which can be activated by a variety of procedures. This enzyme is associated with a cytochrome of the b type. 2. The inhibitors, azide, cyanate, thiocyanate and nitrite, react
L P, Solomonson, B, Vennesland
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Nitrate reductases inEscherichia coli
Antonie van Leeuwenhoek, 1994Escherichia coli expresses two different membrane-bound respiratory nitrate reductases, nitrate reductase A (NRA) and nitrate reductase Z (NRZ). In this review, we compare the genetic control, biochemical properties and regulation of these two closely related enzyme systems.
V, Bonnefoy, J A, Demoss
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Histochemical localization of nitrate reductase
Histochemistry, 1981NADH-dependent nitrate reductase (E.C. 1.6.6.1) was ultrastructurally localized in norflurazon-treated and control soybean cotyledons [Glycine max (L.) Merr.] by a method based upon the increase in osmiophilia due to the formation of an azo dye. The reaction product was observed in small vesicles throughout the cytoplasm.
K C, Vaughn, S O, Duke
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A method for the determination of nitrate reductase
Journal of Biochemical and Biophysical Methods, 1981A procedure for the assay of nitrate reductase based on Szekely's diaminodiphenylsulphone method of nitrate determination (Szekely, E. (1967) Talanta 14, 941-950) is described. The method is simple and sensitive, allowing determination of 1 microgram of nitrate in a volume of 1 ml or less.
R M, Daniel, M P, Curran
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Reductive activation of nitrate reductases
Dalton Transactions, 2005Protein film voltammetry of Paracoccus pantotrophus respiratory nitrate reductase (NarGH) and Synechococcus elongatus assimilatory nitrate reductase (NarB) shows that reductive activation of these enzymes may be required before steady state catalysis is observed.
Field, Sarah J. +9 more
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Activation of nitrate reductase by oxidation
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1972Abstract The activation of the inactive form of the nitrate reductase (NADH: nitrate oxidoreductase, EC 1.6.6.1) present in cell-free extracts of Chlorella vulgaris Beijerinck requires an oxidizing agent. Ferricyanide causes conversion of the proenzyme to active enzyme within a few minutes, even at 0°C. Molecular oxygen causes a slow activation which
K, Jetschmann +2 more
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[Induced activity of nitrate reductase by nitrate and cloning of nitrate reductase gene].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2010Excessive nitrate accumulated in plants affects vegetable quality severely and excessive nitrate ingestion would do harm to human health. Assimilatory NADH: nitrate reductase (NR, EC 1.6.6.1), a complex Mo-pterin-, cytochrome b(557)- and FAD-containing protein, catalyzes the regulated and rate-limiting step in the utilization of inorganic nitrogen by ...
Li-Qun, Wang +3 more
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Bacterial dimethyl sulphoxide reductases and nitrate reductases
Biochemical Society Transactions, 1991interactions which can be done when more structural information is available, but they do provide order of magnitude estimates of interaction strengths. Evidently, considerable rearrangement of charge driven by reduction significantly raises the effective dielectric. It seems likely that centres S-2 and FR-2 are apparently low-potential clusters merely
McEwan, Alastair G. +6 more
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Nitrate reductase for nitrate analysis in water
Environmental Chemistry Letters, 2006Nitrate analysis in water is one of the most frequently applied methods in environmental chemistry. Current methods for nitrate are generally based on toxic substances. Here, we show that a viable alternative method is to use the enzyme nitrate reductase.
Wilbur H. Campbell +2 more
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The bacterial nitrate reductases: EPR studies on nitrate reductase A from Micrococcus denitrificans
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1972EPR studies on nitrate reductase indicate at 80 °K a signal at g=1.985 and g=2.045 attributable to Mo(V). At 15 °K a signal is observed at g=2.016 due to Fe(III) in an unknown symmetry. The reaction of nitrate reductase with nitrate, nitrite or azide results in changes of the Mo(V) signal.
P, Forget, D V, Dervartanian
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