Results 71 to 80 of about 7,506,338 (209)

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Effects of the time of application and the form of nitrogen on two maize inbred lines seed yield [PDF]

Selekcija i Semenarstvo, 2005
The study was carried out in the experimental filed of the Maize Research Institute, Zemun Polje, on calcareous chernozem in the period 2001-2003. The traits of two maize inbred lines (L1-FAO 400 and L2-FAO 600) were observed in dependence on the time of
Hojka Zdravko
doaj  

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Occurrence Characteristics and Release Potential of Different Forms of Nitrogen in Surface Sediments of the Nanshun Lian'anwei Network Water System in Shunde District

Renmin Zhujiang
To reveal the endogenous release risk of nitrogen in surface sediments of river network systems in southern China, this study focused on a typical urbanized area, namely the Nanshun Lian'anwei water system in Shunde District.
ZHANG Bo, YUAN Bo, LI Yuan, YAO Pujing, ZHANG Xifeng, GUO Mengjing   +5 more
doaj   +2 more sources

Host Genotype and Nitrogen Form Shape the Root Microbiome of Pinus radiata

, 2018
A central challenge in community ecology is understanding the role that phenotypic variation among genotypes plays in structuring host-associated communities.
Love, Jonathan, Meason, Dean F, Turnbull, Matthew H, Clinton, Peter W, Gallart, Marta, Xue, Jianming, Adair, Karen L, Turnbull, Matthew H.,, Sveriges lantbruksuniversitet., Adair, Karen L.,, Swedish University of Agricultural Sciences., Xue, Jianming,, Meason, Dean F.,, Clinton, Peter W.,, Gallart, Marta,, Love, Jonathan,   +15 more
core   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Long-term maize-soybean crop rotation: impacts on soybean yield, soil microbiota and nitrogen dynamics

Frontiers in Plant Science
AimsSoil nitrogen is recognized as a vital nutrient influencing soybean growth and yield. Hence, a comprehensive understanding of the intricate connections between shifts in nitrogen patterns and the behaviors of soil microbial communities and crucial ...
Liqiang Zhang, Liqiang Zhang, Jiamin Yin, Wenxiu Ji, Jingcheng Zhao, Zhuo Xin, Demin Rao, Fangang Meng, Jinhu Cui, Wei Zhang, Hongyan Zhao   +10 more
doaj   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

The Nitrate-First, Ammonium-Later Strategy in Potato: Implications of Nitrogen Timing, Form, and Soil Transformation

Agronomy
Potato nitrogen (N) demand varies with developmental stage rather than remaining uniformly high throughout the season. This review re-examines the “nitrate-first, ammonium-later” strategy by separating total N amount, N-supply timing, N form, and soil N ...
Jing Yu, Xiaohua Shi, Yonglin Qin, Li Li, Yang Chen, Liguo Jia, Mingshou Fan   +6 more
doaj   +1 more source