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Quantifying Nitrogen Uptake Rates of Maize Roots Using Stable Isotopes
Cold Spring Harbor ProtocolsNitrogen is an essential element for plant growth and development; however, application of nitrogen (N)-based fertilizers comes with a high environmental cost. This includes the energy required for production, volatilization from fields, and runoff or leaching to waterways triggering algal blooms.
Findimila Dio Ishaya, Amanda Rasmussen
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Stable isotopes of carbon and nitrogen in soil ecological studies
Biology Bulletin, 2007The development of stable isotope techniques is one of the main methodological advances in ecology of the last decades of the 20th century. Many biogeochemical processes are accompanied by changes in the ratio between stable isotopes of carbon and nitrogen (12C/13C and 14N/15N), which allows different ecosystem components and different ecosystems to be
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Fractionation of nitrogen stable isotopes by ion exchange chromatography
Journal of Radioanalytical and Nuclear Chemistry, 2003The ion exchange chromatography displacement technique was used for the enrichment of 15N. The runs were conducted in laboratory and bench scales using two systems of columns filled with Wofatit KPS ion exchange resin (medium porosity type). Ammonia NH4+/NH3 aq. was chosen as the isotopic exchange system.
Aguilera Y +4 more
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SEPARATION OF STABLE NITROGEN ISOTOPES BY ION EXCHANGE CHROMATOGRAPHY
Solvent Extraction and Ion Exchange, 2002ABSTRACT The ion exchange chromatography technique was used for the enrichment of nitrogen 15 (15N). A set of columns filled with the ion exchange resin Wofatit KPS (medium porosity type) with 5.4 cm I.D. and 1.5 m height was set up. Ammonia NH4 +/NH3 aq. was chosen as the isotopic exchange system.
C. Y. Aguilera +3 more
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Bulletin of Environmental Contamination and Toxicology, 2020
Maolan Wang +4 more
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Maolan Wang +4 more
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Deciphering Soil Nitrogen Biogeochemical Processes Using Nitrogen and Oxygen Stable Isotopes
2019Variations in stable isotope abundances of nitrogen (δ15N) and oxygen (δ18O) of nitrate are a useful tool for determining sources of nitrate as well as understanding the transformations of nitrogen within soil (Chapter 2). Various sources of nitrate are known to display distinctive isotopic compositions, while nitrogen transformation processes ...
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Metabolic Labeling of Microorganisms with Stable Heavy Nitrogen Isotopes (15N)
2018Quantitative Proteomics based on stable isotope labeling by 15N is a versatile method in determination of changes in microbial protein expression profiles. Once set up, the workflow relies on defined chemical or commercially available complex media leading to universal heavy labeling by the stable isotope 15N.
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Carbon, Nitrogen and Oxygen Stable Isotope Ratios in Chitin
2010Stable isotope ratios in chitin are firmly imprinted during biopolymer biosynthesis and reflect dietary, metabolic, and environmental influences. Chitin is a chemically complex amino sugar biopolymer that also includes non-amino-sugar moieties with contrasting isotopic compositions.
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