Results 81 to 90 of about 960,188 (308)

Microbial protein out of thin air : fixation of nitrogen gas by an autotrophic hydrogen-oxidizing bacterial enrichment [PDF]

, 2020
For the production of edible microbial protein (MP), ammonia generated by the Haber-Bosch process or reclaimed ammonia from waste streams is typically considered as the nitrogen source.
Bernaerts, Kristel, Boeckx, Pascal, Boon, Nico, Clauwaert, Peter, Ghesquiere, Justien, Hu, Xiaona, Kerckhof, Frederiek-Maarten   +6 more
core   +2 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Designing a Sustainable Temporary Grassland System by Monitoring Nitrogen Use Efficiency

Agronomy, 2020
Assessing the efficiency of nitrogen application is a valuable first step in establishing an optimum N fertilizer management plan. The aim of this study was to elaborate a sustainable N management system for sown grassland installed in temperate regions,
Anamaria Mălinas, Ioan Rotar, Roxana Vidican, Vasile Iuga, Florin Păcurar, Cristian Mălinas, Cristina Moldovan   +6 more
doaj   +1 more source

Microbial ecosystem constructed in water for successful organic hydroponics [PDF]

, 2008
Conventional hydroponics systems generally use only chemical fertilisers, not organic ones, since there are no microbial ecosystems present in such systems to mineralise organic compounds to inorganic nutrients.
Hiromi Ohmori, Makoto Shinohara, Yoichi Uehara   +2 more
core   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Potato growth, nitrogen balance, quality, and productivity response to water-nitrogen regulation in a cold and arid environment

Frontiers in Plant Science
BackgroundThe pervasively imprudent practices of irrigation and nitrogen (N) application within Oasis Cool Irrigation zones have led to significant soil nitrogen loss and a marked decrease in water and nitrogen use efficiency.MethodsTo address this ...
Dandan Su, Dandan Su, Hengjia Zhang, Anguo Teng, Changlong Zhang, Lian Lei, Yuchun Ba, Chenli Zhou, Fuqiang Li, Xietian Chen, Zeyi Wang   +10 more
doaj   +1 more source

Breeding for improved nitrogen use efficiency in oilseed rape [PDF]

, 2008
Oilseed rape has a high requirement for nitrogen (N) fertiliser relative to its seed yield. This paper uses published and unpublished work to explore the extent to which the N use efficiency (seed yield ÷ N supply) of oilseed rape could be improved ...
Berry, P. M. (Peter M.), Foulkes, John, Spink, J. H. (John H.), Teakle, Graham R., White, Philip J.   +4 more
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

An Analysis of Allocative Efficiency of Wheat Growers in Northern Pakistan [PDF]

Restricted translog production model is used to estimate the allocative efficiency of wheat growers in Peshawar Valley. It is found that average allocative efficiency is 72 percent.
Dilawar Khan, Muhammad Bashir
core