Results 251 to 260 of about 4,164,802 (316)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Hybrid sequencing reveals incompleteness of the H37Rv reference genome and highlights lineage-specific genomic divergence in <i>Mycobacterium tuberculosis</i>. [PDF]

Microb Genom
Chen H, Li X, Chen R, Zhang M, Zhu Y, Wu K, Lu Y, Wang X, Che Y, Liu Z.   +9 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Preoperative PNI-dNLR Score as Predicting Long-Term Survival in Older Adults Undergoing Radical Gastrectomy for Gastric Cancer. [PDF]

Clin Interv Aging
Chen XL, Zhang XM, Lin ZX, Huang XL, Chen YZ, Lin WZ, Zheng CY, Lin W.   +7 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Wild Pedigree exploreR (wpeR): Streamlined Analysis and Visualization of Wild Pedigrees in Time and Space. [PDF]

Mol Ecol Resour
Simčič G, Skrbinšek T.
europepmc   +1 more source

Verification of harmonized reference intervals in Croatia: is it time for a change? [PDF]

Biochem Med (Zagreb)
Friščić I, Burazer I, Radeljak A, Perkov S, Kardum Paro MM.   +4 more
europepmc   +1 more source

Sexual Orientation-Related Discrimination Among LGB+ Medical Students With Disabilities.

JAMA Netw Open
Sheets ZC, Nguyen M, Lopez JKM, Addams A, Moreland CJ, Boatright D, Meeks LM.   +6 more
europepmc   +1 more source

The evolution of life size Barbie and Ken: Are they any closer to reality? [PDF]

PLoS One
Grafenauer S, Durey B, Norton K.
europepmc   +1 more source