Results 151 to 160 of about 23,452 (277)

A neuromorphic processor with on-chip learning for beyond-CMOS device integration. [PDF]

Nat Commun
Greatorex H, Richter O, Mastella M, Cotteret M, Klein P, Fabre M, Rubino A, Soares Girão W, Chen J, Ziegler M, Bégon-Lours L, Indiveri G, Chicca E.   +12 more
europepmc   +1 more source

Clinical performance of the urine‐based TERT promoter AbsoluteQ Digital PCR for non‐invasive detection of bladder cancer

Molecular Oncology, EarlyView.
A urine‐based digital PCR assay targeting two hotspot TERT promoter variants detected bladder cancer with high sensitivity and no false positives in this case–control cohort. The streamlined AbsoluteQ workflow outperformed Sanger sequencing and supports non‐invasive molecular testing for bladder cancer detection.
Anna Nykel, Izabela Kubiak, Lena Rutkowska, Żaneta Kasprzyk, Łukasz Kępczyński, Michał Bednarek, Dariusz Sobieraj, Jacek Wilkosz, Piotr Kania, Adam Jędrzejczyk, Bogdan Kałużewski, Agnieszka Gach, Tadeusz Kałużewski   +12 more
wiley   +1 more source

microRNAs for qPCR Normalization Under Morphofunctional Conditions in Bovine Sperm (Bos taurus). [PDF]

Mol Reprod Dev
de Souza LP, Nunes LS, Salvi LC, Gonçalves LDS, Reis LFVD, Acosta IB, Corcini CD, Junior ASV, Barreto FG, Vieira MB, Silveira DC, Vieira JM, Ilha GF, Domingues WB, Campos VF.   +14 more
europepmc   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Causes and consequences of experimental variation in Nicotiana benthamiana transient expression. [PDF]

Nat Commun
Tang SN, Szarzanowicz MJ, Lanctot A, Sirirungruang S, Kirkpatrick LD, Drako K, Alamos S, Cheng L, Waldburger LM, Liu S, Huang L, Kazaz S, Akyuz Turumtay E, Baidoo E, Eudes A, Thompson MG, Shih PM.   +16 more
europepmc   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

A fluorescent sensor for real-time monitoring of DPP8/9 reveals crucial roles in immunity and cancer. [PDF]

Life Sci Alliance
Weiss K, Agarkova Y, Zwosta A, Hoevel S, Himmelreich AK, Shumanska M, Etich J, Poschmann G, Brachvogel B, Bogeski I, Mielenz D, Riemer J.   +11 more
europepmc   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

The MCTOT app: A publicly available tool for statistical cycle-to-threshold analysis and inference of informative but uncertainly determined qPCR data. [PDF]

PLoS One
Zhuang W, Liu J.
europepmc   +1 more source

Tumor‐stromal crosstalk and macrophage enrichment are associated with chemotherapy response in bladder cancer

FEBS Open Bio, EarlyView.
Chemoresistance in bladder cancer: Macrophage recruitment associated with CXCL1, CXCL5 and CXCL8 expression is characteristic of Gemcitabine/Cisplatin (Gem/Cis) Non‐Responder tumors (right side) while Responder tumors did not show substantial tumor‐stromal crosstalk (left side). All biological icons are attributed to Bioicons: carcinoma, cancerous‐cell‐
Sophie Leypold, Janik Riese, Lancelot Seillier, Mark Kühnel, Julia Pannhausen, Charlotte O. J. Fröhlich, Christian Martin, Peter Boor, Matthias Saar, Danny D. Jonigk, Nadine T. Gaisa, Michael Rose   +11 more
wiley   +1 more source