Identification and subcellular localization of the chaperonin NbCCTβ in Nosema bombycis
GeneNosema bombycis, the causative agent of pebrine disease, poses a significant threat to the silkworm industry due to its negative impact on silkworm health and productivity. The chaperonin-containing tailless complex polypeptide (CCT) plays a crucial role in protein folding, and its β subunit (CCTβ) is essential for the proper folding of cytoskeletal ...
Shengyan, Xiao +6 more
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Preliminary genomic characterization of microsporidian Nosema bombycis
World Journal of Microbiology and Biotechnology, 2000In order to characterize the genome of Nosema bombycis, the techniques of karyotyping, pulsed field gel electrophoresis, and polymerase chain reaction were applied. Nosema genomic DNA moved as 23 kb fragment on a standard agarose gel. The karyotype showed four chromosomes, the molecular karyotyping by pulsed field gel electrophoresis also showed four ...
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SWP25, a novel protein associated with the Nosema bombycis endospore.
The Journal of eukaryotic microbiology, 2011Microsporidia are eukaryotic, obligate intracellular, spore-forming parasites. The resistant spores, which harbor a rigid cell wall, are critical for their host-to-host transmission and persistence in the environment. The spore wall comprises two major layers: the exospore and the endospore.
Zhengli, Wu +4 more
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Nosema bombycis Replication in a Manduca sexta Cell Line
The Journal of Parasitology, 1984Nineteen B. glabrata (NIH-PR 1 strain) which had been individually exposed to 5-10 S. mansoni miracidia 58 days previously were placed in the apparatus. The average size of these snails was 15.2 mm + 1.65 mm. The snails were allowed to acclimatize to the apparatus for 3 days. Following this period, cercarial emergence from each snail was measured every
D. A. Streett, D. E. Lynn
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Some properties of ribosomes from the sporoplasm of Nosema bombycis
Journal of Invertebrate Pathology, 1968Abstract Ribosomes were separated from the sporoplasm of the microsporidian Nosema bombycis , and some properties were studied by using a sucrose density gradient. The ribosomes were mainly monoribosomes with a sedimentation coefficient resembling that of Escherichia coli (= 70 S), and in the medium containing Mg 2+ ions of less than 0.1m m ...
R. Ishihara, Y. Hayashi
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Nosema bombycis: A remarkable unicellular parasite infecting insects
Journal of Eukaryotic MicrobiologyAbstractMicrosporidia are opportunistic fungal‐like pathogens that cause microsporidiosis, which results in significant economic losses and threatens public health. Infection of domesticated silkworms by the microsporidium Nosema bombycis causes pébrine disease, for which this species of microsporidia has received much attention.
Qingyuan Huang +4 more
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Divergence of a Tandem Duplication of Manganese Superoxide Dismutase in Nosema bombycis
Journal of Eukaryotic Microbiology, 2017AbstractManganese superoxide dismutase (MnSOD) is a key enzyme in the protection of cells from oxidative stress. A tandem duplication of the MnSOD gene (NbMnSOD1 and NbMnSOD2) in the genome of Nosema bombycis, a parasite of the silkworm Bombyx mori, was previously identified.
Ying Wang +11 more
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Analyses of the Ribosomal DNA Region in Nosema bombycis NIS 001
Journal of Eukaryotic Microbiology, 2004ABSTRACT Ribosomal DNA (rDNA) containing small subunit (SSU) rDNA and both flanking regions in the entomopathogenic microsporidian Nosema bombycis NIS 001 was amplified from genomic DNA with a primer set based on the sequence of an inverse polymerase chain reaction (PCR) ‐derived fragment. In this fragment, SSU rDNA was divided by a 618‐bp insert at nt
Kazuhiro, Iiyama +4 more
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In vitro expression and functional characterization of NPA motifs in aquaporins of Nosema bombycis
Parasitology Research, 2018Nosema bombycis contains functional aquaporins (NbAQPs), which are key targets for exploring the mechanism of N. bombycis infection; however, the regulation of these NbAQPs remains unknown. The two highly conserved asparagine-proline-alanine sequences (NPA motifs) play important roles in AQP biogenesis. As part of this study, we constructed a series of
Gong Chen +6 more
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Immunological characterization of spore proteins of the microsporidian Nosema bombycis
World Journal of Microbiology and Biotechnology, 1999The spore proteins of the microsporidian, Nosema bombycis, from the silkworm, Bombyx mori were analysed by SDS-polyacrylamide gel electrophoresis. The western blot analysis using total antibodies showed major antigenic peptides of sizes 94, 68, 45, 31, 28, 17 and 12.5 kDa.
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