Nosema bombycis Replication in a Manduca sexta Cell Line
The Journal of Parasitology, 1984Nineteen B. glabrata (NIH-PR 1 strain) which had been individually exposed to 5-10 S. mansoni miracidia 58 days previously were placed in the apparatus. The average size of these snails was 15.2 mm + 1.65 mm. The snails were allowed to acclimatize to the apparatus for 3 days. Following this period, cercarial emergence from each snail was measured every
D. A. Streett, D. E. Lynn
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Nosema bombycis: A remarkable unicellular parasite infecting insects
Journal of Eukaryotic MicrobiologyAbstractMicrosporidia are opportunistic fungal‐like pathogens that cause microsporidiosis, which results in significant economic losses and threatens public health. Infection of domesticated silkworms by the microsporidium Nosema bombycis causes pébrine disease, for which this species of microsporidia has received much attention.
Qingyuan Huang +4 more
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The genomic survey of Tc1-like elements in the silkworm microsporidia Nosema bombycis
Acta Parasitologica, 2019Microsporidia Nosema bombycis is the destructive pathogen in the production of sericulture. The Tc1/mariner elements belong to important component of DNA transposon.The genomic data of N. bombycis and related Nosema species were screened to identify the Tc1-like elements and analyzed the phylogenetic relationship, based on bioinformational analysis ...
Huali Song +4 more
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Divergence of a Tandem Duplication of Manganese Superoxide Dismutase in Nosema bombycis
Journal of Eukaryotic Microbiology, 2017AbstractManganese superoxide dismutase (MnSOD) is a key enzyme in the protection of cells from oxidative stress. A tandem duplication of the MnSOD gene (NbMnSOD1 and NbMnSOD2) in the genome of Nosema bombycis, a parasite of the silkworm Bombyx mori, was previously identified.
Ying Wang +11 more
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Analyses of the Ribosomal DNA Region in Nosema bombycis NIS 001
Journal of Eukaryotic Microbiology, 2004ABSTRACT Ribosomal DNA (rDNA) containing small subunit (SSU) rDNA and both flanking regions in the entomopathogenic microsporidian Nosema bombycis NIS 001 was amplified from genomic DNA with a primer set based on the sequence of an inverse polymerase chain reaction (PCR) ‐derived fragment. In this fragment, SSU rDNA was divided by a 618‐bp insert at nt
Kazuhiro, Iiyama +4 more
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Immunological characterization of spore proteins of the microsporidian Nosema bombycis
World Journal of Microbiology and Biotechnology, 1999The spore proteins of the microsporidian, Nosema bombycis, from the silkworm, Bombyx mori were analysed by SDS-polyacrylamide gel electrophoresis. The western blot analysis using total antibodies showed major antigenic peptides of sizes 94, 68, 45, 31, 28, 17 and 12.5 kDa.
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In vitro expression and functional characterization of NPA motifs in aquaporins of Nosema bombycis
Parasitology Research, 2018Nosema bombycis contains functional aquaporins (NbAQPs), which are key targets for exploring the mechanism of N. bombycis infection; however, the regulation of these NbAQPs remains unknown. The two highly conserved asparagine-proline-alanine sequences (NPA motifs) play important roles in AQP biogenesis. As part of this study, we constructed a series of
Gong Chen +6 more
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Functional characterization of trehalases 1, 2, and 4 in Nosema bombycis (microsporidia)
Microbial PathogenesisPebrine caused by Nosema bombycis infection is one of the most threatening diseases that affect sericulture. Trehalases play important roles in the lives of organisms, such as spore germination, insect flight, and stress resistance. The genome of N. bombycis has four trehalase genes, but their functions in the infection and development of N.
Chenxiao Dong +8 more
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[Characterization and analysis of mitochondrial protein frataxin in Nosema bombycis].
Wei sheng wu xue bao = Acta microbiologica Sinica, 2009Frataxin protein is a component of Fe-S clusters and closely related to metabolism of mitochondria. We identified an integrity mitochondrial protein frataxin gene (Nbfra), analyzed its phylogenetic relationship, and confirmed the transcriptase activity of Nbfra in N. bombycis.We analyzed the sequence of the second structure, gene location in genome and
Junhua, Hu +7 more
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Strategies for diagnosing Nosema bombycis (Microsporidia: Nosematidae); the agent of pebrine disease
Molecular and Biochemical ParasitologyPebrine disease, caused by Nosema bombycis (N. bombycis), is the most important pathogen known to the silk industry. Historical evidence from several countries shows that the outbreaks of pebrine disease have largely caused the decline of the sericulture industry.
Masoumeh, Bagheri +2 more
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