EPR spectroscopy 80 years after its discovery. [PDF]
Goldfarb D +5 more
europepmc +1 more source
Adenosine triphosphate as a modulator of protein interactions and stability
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
Correction to "Insights into the regulatory roles of LIKE-HETEROCHROMATIN PROTEIN 1 and its targeting to different nuclear compartments modulated by NLS and the conserved domains in the moss Physcomitrium patens". [PDF]
europepmc +1 more source
TRiC/CCT chaperonin governs RNA polymerase II activity in the nucleus to support RNA homeostasis. [PDF]
Gvozdenov Z +7 more
europepmc +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
HO-1 Nuclear Interactome Implications in Neuroendocrine Transdifferentiation in Prostate Cancer. [PDF]
Seniuk R +14 more
europepmc +1 more source
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley +1 more source
Geometric mechanogenomics: engineering boundary conditions for deterministic cell fate control. [PDF]
Taghizadeh M, Taghizadeh A, Kim HS.
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Nicotinamide Mononucleotide Adenylyltransferase 1 and NAD<sup>+</sup> Homeostasis in Neuroprotection and Aging. [PDF]
Sun Y, Li B, Qian Z.
europepmc +1 more source

