Results 41 to 50 of about 575,297 (245)

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Direct determination of aluminum in low-enriched UAlx targets (UAlx-Al) by Inductively Coupled Plasma Optical Emission Spectrometry

Brazilian Journal of Radiation Sciences, 2021
The production of molybdenum-99 (99Mo) using low-enriched uranium targets (< 20% 235U) dispersed in aluminum (UAlx) is a very promising strategy towards the independence in 99Mo local production.
Sabine Neusatz Guilhen, Alexandre Luiz de Souza, Marycel Elena Barboza Cotrim, Maria Aparecida Faustino Pires   +3 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

A PROPOSAL OF PROCESS VALIDATION IN THE IMPLEMENTATION OF GOOD MANUFACTURING PRACTICES IN BRACHYTHERAPY SOURCES PRODUCTION

Brazilian Journal of Radiation Sciences, 2021
New laboratories for brachytherapy sources production are being implemented in our facility at IPEN, in São Paulo. A great challenge implementing a production laboratory is to comply with the Good Manufacturing Practices (GMPs), which involves process ...
Tatyana Spinosa Baptista, Anselmo Feher Feher, Bruna Teiga Rodriguez, Carlos Alberto Zeituni, João Augusto Moura, Maria Elisa Rostelato   +5 more
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Calibration coefficients of epitaxial diodes used in diagnostic radiology and computed tomography beams

Brazilian Journal of Radiation Sciences
The assessment of the calibration coefficients of an epitaxial diode, previously characterized for diagnostic radiology (RQR qualities) and computed tomography (RQT qualities) dosimetry, is reported in this work. The diode, with an n-type epitaxial layer
Josemary A. C. Gonçalves, Patrícia de Lara Antonio, Linda V. E. Caldas, Carmen C. Bueno   +3 more
doaj   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Determination of α+nucleus optical potential relevant for p-process study from the natIn(α, α) elastic scattering [PDF]

EPJ Web of Conferences
The knowledge of alpha optical potential is important for determining the nuclear reaction rates. natIn(α, α)natIn elastic scttering cross-sections have been measured at energies Elab = 26 and 29 MeV above the coulomb barrier.
Basak Dipali, Bar Tanmoy, Sahoo Lalit Kumar, Saha Sukhendu, Rana Tapan Kumar, Bhattacharya Chandana, Manna Santu, Kundu Samir, Sahoo Jayanta Kr, Meena Jaikiran, Saha Amiya Kr, Basu Chinmay   +11 more
doaj   +1 more source

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

Byproduct-based zeolite type A as absorbent material for decontamination of simulated radioactive wastewater

Brazilian Journal of Radiation Sciences
The secure disposal of radioactive wastewater, a waste from nuclear operations, presents a significant challenge due to the presence of hazardous radionuclides like cesium.
M.S. Araujo, D.L. Costa-Silva, S.R. Mello Castanho, D.A. Fungaro   +3 more
doaj   +1 more source