Results 101 to 110 of about 426,270 (312)

Quelle {é}thique pour quelle IA ? [PDF]

open access: yesarXiv
This study proposes an analysis of the different types of ethical approaches involved in the ethics of AI, and situates their interests and limits. First, the author introduces to the contemporary need for and meaning of ethics. He distinguishes it from other registers of normativities and underlines its inadequacy to formalization.
arxiv  

Protocol for live-cell super-resolution imaging of transport of pre-ribosomal subunits through the nuclear pore complex

open access: yesSTAR Protocols
Summary: Here, we present a protocol for single-molecule super-resolution imaging of the nuclear export of pre-ribosomal subunits pre-40S and pre-60S through nuclear pore complexes. We describe steps for plating cells and co-transfecting cells.
Mark Tingey   +3 more
doaj  

Protocol for electrical modulation of ERK signaling dynamics in cell lines

open access: yesSTAR Protocols, 2022
Summary: Here, we describe a protocol for modulating the dynamics of the extracellular signal-regulated kinases (ERK) pathway in a customized alternating current (AC) electric field stimulation chamber.
Kan Zhu   +6 more
doaj  

Protocol for FRAP-based estimation of nuclear import and export rates in single yeast cells

open access: yesSTAR Protocols
Summary: Here, we present a protocol for estimating nuclear transport parameters in single cells. We describe steps for performing four consecutive fluorescence recovery after photobleaching experiments, fitting the obtained data to an ordinary ...
Lucía Durrieu   +2 more
doaj  

Protocol to image and quantify nucleocytoplasmic transport in cultured cells using fluorescent in situ hybridization and a dual reporter system

open access: yesSTAR Protocols, 2022
Summary: Nucleocytoplasmic transport (NCT) plays critical roles in maintaining cellular homeostasis. Here, we present a protocol to measure NCT for both transcript and protein cargos in cultured cells.
Haochen Cui   +5 more
doaj  

Mitochondrial DNA disorders in neuromuscular diseases in diverse populations

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
Abstract Neuromuscular features are common in mitochondrial DNA (mtDNA) disorders. The genetic architecture of mtDNA disorders in diverse populations is poorly understood. We analysed mtDNA variants from whole‐exome sequencing data in neuromuscular patients from South Africa, Brazil, India, Turkey and Zambia. In 998 individuals, there were two definite
Fei Gao   +34 more
wiley   +1 more source

Whole-mount immunofluorescent labeling of the adult fly retina

open access: yesSTAR Protocols, 2022
Summary: The adult Drosophila compound eye is an ideal in vivo model for studying biological questions. However, light microscopy of this tissue requires cumbersome embedding and sectioning.
Min-Chun Hsiao   +5 more
doaj  

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