Results 11 to 20 of about 663,366 (347)

In situ Nuclear Matrix preparation in Drosophila melanogaster enabling genetic analysis of the nuclear architecture

open access: yesSTAR Protocols, 2022
Summary: Nuclear Matrix (NuMat) is a biochemically defined entity that provides us with a snapshot of the features of the nuclear architecture. Here, we present a protocol to isolate and visualize NuMat in situ in the intact embryo or tissues of ...
Rashmi U. Pathak   +3 more
doaj   +1 more source

An RNA-immunoprecipitation protocol to identify RNAs associated with RNA-binding proteins in cytoplasmic and nuclear Drosophila head fractions

open access: yesSTAR Protocols, 2022
Summary: RNA-binding proteins (RBPs) are multifunctional proteins that shuttle between the nucleus and the cytoplasm where they assemble with target RNAs to form multi-molecular complexes.
Marjorie Heim   +2 more
doaj   +1 more source

Tandem immuno-purification of affinity-tagged proteins from mouse testis extracts for MS analysis

open access: yesSTAR Protocols, 2022
Summary: Here, we describe a protocol for extraction and tandem immunoprecipitation of the cytoplasmic and nuclear proteins from mouse testis for mass spectrometry. This protocol has been applied to knockin mice that express a meiotic protein of interest
Naoki Tani   +2 more
doaj   +1 more source

Imaging and quantification of apical microvilli in the syncytial blastoderm of Drosophila embryos

open access: yesSTAR Protocols, 2022
Summary: The syncytial Drosophila blastoderm embryo contains apical microvilli with filamentous actin that are remodeled during nuclear division cycles 10–13.
Debasmita Mitra   +3 more
doaj   +1 more source

Differential affinity purification and mass spectrometry analysis of two nuclear pore complex isoforms in yeast S. cerevisiae

open access: yesSTAR Protocols, 2023
Summary: Two isoforms of the nuclear pore complex (NPC) have been identified in the yeast S. cerevisiae, which coexist at the periphery of the nucleus and differ by the presence or absence of a nuclear basket.
Pierre Bensidoun   +2 more
doaj   +1 more source

Universalising the TPNW: Challenges and Opportunities

open access: yesJournal for Peace and Nuclear Disarmament, 2021
The Treaty on the Prohibition of Nuclear Weapons (TPNW) is a landmark agreement in the history of the nuclear disarmament movement. It was negotiated against the wishes of the nuclear-armed states and many of their supporters and this context defines the
Nick Ritchie   +1 more
doaj   +1 more source

Liberal democracy and nuclear despotism: two ethical foreign policy dilemmas

open access: yesEthics & Global Politics, 2013
This article advances a critical analysis of John Rawls's justification of liberal democratic nuclear deterrence in the post-Cold War era as found in The Law of Peoples. Rawls's justification overlooked how nuclear-armed liberal democracies
Thomas E. Doyle
doaj   +1 more source

An ethical dimension to accident management and health surveillance

open access: yesEnvironment International, 2021
Many radiation protection actions carry a multitude of direct and indirect consequences that can impact on the welfare of affected populations. Health surveillance raises ethical challenges linked to privacy and data protection, as well as questions ...
Deborah Oughton   +5 more
doaj   +1 more source

Making the invisible visible

open access: yesTATuP – Zeitschrift für Technikfolgenabschätzung in Theorie und Praxis, 2019
Technology assessment (TA) is an analytic and interactive practice that produces evaluative judgments about the societal implications of technology. Despite this distinct evaluative disposition, “normativities” inherent in TA programs and practices often
Federica Lucivero   +2 more
doaj   +1 more source

The Times of Caring in a Nuclear World: Sculpture, Contamination and Stillness

open access: yesArts, 2021
Care takes time. Caring, whether with, for, or about a living being or entity that is more-than-human, disrupts expectations of how a linear, human time should progress.
Wallace Heim
doaj   +1 more source

Home - About - Disclaimer - Privacy