Results 31 to 40 of about 663,366 (347)

Identification of phosphorylation site on PARP1 mediating its cytosolic translocation in virus-infected HeLa cells

STAR Protocols, 2022
Summary: Poly (ADP-ribose) polymerase 1 (PARP1) localization is controlled by its phosphorylation state. Here, we describe a protocol to monitor PARP1 subcellular localization in HSV-1-infected HeLa cells using immunofluorescence microscopy and ...
Fei Wang, Ming Tong Ma, Junfang Xu, Haipeng Liu   +3 more
doaj   +1 more source

The Russo-Ukrainian conflict and the ethics of war of Augustine and Aquinas

Ethic@: an International Journal for Moral Philosophy
This article analyzes the justice of the Russo-Ukrainian War from the views of just war theorists Augustine and Aquinas. Augustine of Hippo, in his war ethics, believed that war is both a consequence of sin and a potential remedy.
Pedro Erik Carneiro
doaj   +1 more source

Protocol for affordable and efficient profiling of nascent RNAs in bread wheat using GRO-seq

STAR Protocols, 2022
Summary: Exorbitant sequencing cost is one of the main obstacles limiting the widespread application of Global Run-On sequencing (GRO-seq) to detect transcriptional activity.
Yan Chen, Jiafu Zhu, Yilin Xie, Zijuan Li, Yijing Zhang, Min Liu, Zhicheng Dong   +6 more
doaj   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Research Priorities for Robust and Beneficial Artificial Intelligence [PDF]

, 2015
Success in the quest for artificial intelligence has the potential to bring unprecedented benefits to humanity, and it is therefore worthwhile to investigate how to maximize these benefits while avoiding potential pitfalls.
Dewey, Daniel, Russell, Stuart, Tegmark, Max   +2 more
core   +2 more sources

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Anticipating the Future in an Uncertain Present: How Individuals Living With Medically Unexplained Cognitive Complaints Value Biomarker‐Based Prognostics

Health Expectations
Introduction In research on Alzheimer's disease (AD) individuals experiencing cognitive problems but not satisfying criteria for mild cognitive impairment (MCI) or AD are referred to as having ‘Subjective Cognitive Decline’ (SCD).
Anne‐Fleur van derMeer, Marjan Knippenberg, Denise Visser, Marianne Boenink   +3 more
doaj   +1 more source

Time-lapse live-cell imaging of pyroptosis by confocal microscopy

STAR Protocols, 2023
Summary: Pyroptosis is a type of regulated necrosis executed by gasdermin. Osmotic cell swelling and membrane perforation are the key features of pyroptosis.
Shuai Jiang, Kunpeng Qin, Li Sun
doaj   +1 more source

The ethics of sociocultural risk research [PDF]

, 2001
In socio-cultural risk research, an epistemological tension often follows if real hazards in the world are juxtaposed against the essentially socially constructed nature of all risk.
Sarre, S, Pidgeon, N, Simmons, P, Henwood, K, Smith, N   +4 more
core   +1 more source

Reduced vascular leakage correlates with breast carcinoma T regulatory cell infiltration but not with metastatic propensity

Molecular Oncology, EarlyView.
A mouse model for vascular normalization and a human breast cancer cohort were studied to understand the relationship between vascular leakage and tumor immune suppression. For this, endothelial and immune cell RNAseq, staining for vascular function, and immune cell profiling were employed.
Liqun He, Chiara Testini, Neda Hekmati, Altea Bonello, Aglaia Schiza, Emmanuel Nwadozi, Mia Phillipson, Carina Strell, Michael Welsh   +8 more
wiley   +1 more source