Results 41 to 50 of about 2,395,767 (232)

Protocol for affordable and efficient profiling of nascent RNAs in bread wheat using GRO-seq

STAR Protocols, 2022
Summary: Exorbitant sequencing cost is one of the main obstacles limiting the widespread application of Global Run-On sequencing (GRO-seq) to detect transcriptional activity.
Yan Chen, Jiafu Zhu, Yilin Xie, Zijuan Li, Yijing Zhang, Min Liu, Zhicheng Dong   +6 more
doaj   +1 more source

Time-lapse live-cell imaging of pyroptosis by confocal microscopy

STAR Protocols, 2023
Summary: Pyroptosis is a type of regulated necrosis executed by gasdermin. Osmotic cell swelling and membrane perforation are the key features of pyroptosis.
Shuai Jiang, Kunpeng Qin, Li Sun
doaj   +1 more source

The Russo-Ukrainian conflict and the ethics of war of Augustine and Aquinas

Ethic@: an International Journal for Moral Philosophy
This article analyzes the justice of the Russo-Ukrainian War from the views of just war theorists Augustine and Aquinas. Augustine of Hippo, in his war ethics, believed that war is both a consequence of sin and a potential remedy.
Pedro Erik Carneiro
doaj   +1 more source

Intra- vs. intergenerational justice tradeoffs in nuclear waste storage (W01)

Safety of Nuclear Waste Disposal, 2023
. We propose a workshop in two parts (approx. 90 min each) to discuss (i) general dilemmas due to the long time range until an operable solution for the waste problem is found and (ii) an ethics-inspired discussion on the extra burden for communities ...
Roman Seidl, Cord Drögemüller, Rosa Sierra   +2 more
semanticscholar   +1 more source

Anticipating the Future in an Uncertain Present: How Individuals Living With Medically Unexplained Cognitive Complaints Value Biomarker‐Based Prognostics

Health Expectations
Introduction In research on Alzheimer's disease (AD) individuals experiencing cognitive problems but not satisfying criteria for mild cognitive impairment (MCI) or AD are referred to as having ‘Subjective Cognitive Decline’ (SCD).
Anne‐Fleur van derMeer, Marjan Knippenberg, Denise Visser, Marianne Boenink   +3 more
doaj   +1 more source

Protocol for analyzing the microstructure of nuclear fiber layers by using structured illumination microscopy coupled with Arivis 4D

STAR Protocols
Summary: Here, we present a protocol for quantitatively assessing the microstructure of nuclear fiber layers by immunolabeling of a nuclear skeleton protein lamin A through structured illumination microscopy (SIM) coupled with an innovative algorithm in ...
Miao Guan, Kewei Li, Xianglong Liu, Xiaoliang Li, Zhenlong Sun   +4 more
doaj   +1 more source

Protocol for imaging nuclear pore complexes on isolated nuclei of zebrafish early embryos by field emission scanning electron microscopy

STAR Protocols, 2023
Summary: Here, we present a protocol to observe the three-dimensional surface of nuclear pore complexes (NPCs) of vertebrate early embryos by field emission scanning electron microscopy (FESEM).
Weimin Shen, Anming Meng
doaj   +1 more source

Protocol for induction and study of DNA double-strand breaks in mammalian cells using PALM microdissection and expansion microscopy

STAR Protocols
Summary: We present a protocol to induce localized DNA double-strand breaks (DSBs) in mammalian monolayer cells, using the PALM MicroBeam microdissector, followed by high-resolution visualization through expansion microscopy (ExM).
Fernando Luna-Maldonado, Diego Oliva-Rico, Alejandro López-Saavedra, Aquetzalli Arenas-Hernández, Clementina Castro-Hernández, Juan Eduardo Rodriguez Gatica, Eloy-Andres Pérez-Yepez, José Díaz-Chávez, Luis A. Herrera   +8 more
doaj   +1 more source

After Fukushima: The precautionary principle revisited

Verbum et Ecclesia, 2012
Etienne de Villiers, more than other theologians, elaborates on basic elements of a Christian ethics of responsibility. He distinguishes between retrospective and prospective responsibility.
Wolfgang Huber
doaj   +1 more source

Evaluation of Urine Polymerase Chain Reaction Test Positivity Rates and the Effectiveness of Positron Emission Tomography in Renal Involvement in Patients with Active COVID-19 Infection: A Prospective and Multidisciplinary Study

Haseki Tıp Bülteni, 2021
Aim:It is known that the Coronavirus disease-2019 (COVID-19) does not only affect the respiratory system in the body, but also affects many vital systems.
Turev Demirtas, Gokhan Sonmez, Omur Mustafa Parkan, Ahmet Tutus, Zeynep Ture, Sevket Tolga Tombul, Orhan Yildiz, Selma Gokahmetoglu, Emrah Kizilay, Abdullah Demirtas   +9 more
doaj   +1 more source