Results 51 to 60 of about 2,395,767 (232)

Ethics and Nuclear Energy

Zbornik Pravnog fakulteta Sveučilišta u Rijeci, 2001
Should the scientist be a morally unbiased person? This is the eternal question asked by many great thinkers interested in science. The answer is hard to find. Scientists are expected to take into consideration the consequences of their actions before they actually start to act.
Nežić, Nadalina, Dodig, Damir
openaire   +2 more sources

Protocol for IPO11 deletion and re-expression in H460 lung cancer cells using CRISPR-Cas9 and plasmid transfection

STAR Protocols, 2023
Summary: Exploring the essential role of Importin 11 (IPO11) in the nuclear translocation of its potential cargo proteins requires an efficient means of IPO11 deletion and re-expression.
Yarui Zhang, Guomin Ou, Zhou Zhou, Jingdong Wang, Hongying Yang   +4 more
doaj   +1 more source

Protocol for constructing a hierarchical host-guest supramolecular self-assembly system in water

STAR Protocols, 2023
Summary: Here, we present a protocol for the construction of a hierarchical host-guest supramolecular self-assembly system in water. We describe steps for determining the binding levels and capturing the morphologies of hierarchical self-assembly.
Xiao Chen, Xu-Man Chen, Quan Li
doaj   +1 more source

Protocol for live-cell super-resolution imaging of transport of pre-ribosomal subunits through the nuclear pore complex

STAR Protocols
Summary: Here, we present a protocol for single-molecule super-resolution imaging of the nuclear export of pre-ribosomal subunits pre-40S and pre-60S through nuclear pore complexes. We describe steps for plating cells and co-transfecting cells.
Mark Tingey, Samuel L. Junod, Coby Rush, Weidong Yang   +3 more
doaj   +1 more source

Protocol for FRAP-based estimation of nuclear import and export rates in single yeast cells

STAR Protocols
Summary: Here, we present a protocol for estimating nuclear transport parameters in single cells. We describe steps for performing four consecutive fluorescence recovery after photobleaching experiments, fitting the obtained data to an ordinary ...
Lucía Durrieu, Alan Bush, Alejandro Colman-Lerner   +2 more
doaj   +1 more source

Protocol for nucleo-cytoplasmic fractionation of mammalian cells to study protein translocation via immunoblotting

STAR Protocols
Summary: Studying protein translocation is essential for understanding protein function and regulatory mechanisms, including the activity of transcription factors.
Adhikarimayum Lakhikumar Sharma, Eddy Zhu, Rithika Padyala, Kavya Parikh, Mudit Tyagi   +4 more
doaj   +1 more source

FOXO3a as a sensor of unilateral nerve injury in sensory neurons ipsilateral, contralateral and remote to injury

Neural Regeneration Research, 2020
Emerging evidence supports that the stress response to peripheral nerve injury extends beyond the injured neuron, with alterations in associated transcription factors detected both locally and remote to the lesion. Stress-induced nuclear translocation of
Jovan C.D. Hasmatali, Jolly De Guzman, Jayne M Johnston, Hossein Noyan, Bernhard H Juurlink, Vikram Misra, Valerie M.K. Verge   +6 more
doaj   +1 more source

Protocol to analyze Drosophila intestinal tumor cellular heterogeneity using immunofluorescence imaging and nuclear size quantification

STAR Protocols
Summary: Drosophila intestinal tumors show an extended cellular heterogeneity. We devise a protocol to assess tumor cell heterogeneity by employing nuclear size measurement and immunofluorescence-based cell lineage analysis.
Inez Keiko Arlyne Pranoto, Young V. Kwon
doaj   +1 more source

Protocol to evaluate the antiviral effect of FDA-approved drugs against dengue virus in Huh7 cells and AG129 mice

STAR Protocols
Summary: Finding an effective therapy against diseases caused by flaviviruses remains a challenge. Here, we present a protocol to test Food and Drug Administration-approved drugs that inhibit host nuclear protein import, promoting a reduction of dengue ...
Selvin Noé Palacios-Rápalo, Jonathan Hernández-Castillo, Carlos Daniel Cordero-Rivera, Magda Lizbeth Benítez-Vega, Luis Adrián De Jesús-González, José Manuel Reyes-Ruiz, Carlos Noe Farfan-Morales, Juan Fidel Osuna-Ramos, Arely M. Gonzalez-Gonzalez, Raymundo Cruz, Rosa María del Ángel   +10 more
doaj   +1 more source

The Hidden Curriculum Challenges in Learning Professional Ethics Among Iranian Medical Students: A Qualitative Study

Advances in Medical Education and Practice, 2020
Yahya Safari,1 Alireza Khatony,2 Mohammad Rasoul Tohidnia3 1Research Center for Environmental Determinants of Health (RCEDH), Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran; 2Clinical Research Development Center, Imam Reza ...
Safari Y, Khatony A, Tohidnia MR
doaj