Results 61 to 70 of about 426,270 (312)

Immunofluorescence protocol for localizing protein targets in brain tissue from diverse model and non-model mammals

STAR Protocols, 2023
Summary: Previous immunostaining protocols are highly specific for model organisms and often not suitable for diverse specimens that are non-perfused and over-fixed (i.e., tissues sitting in fixatives for months/year).
Caterina Ciani, Giulio Pistorio, Marika Mearelli, Carmen Falcone   +3 more
doaj  

Comment on: "The interaction of neutrons with 7Be at BBN temperatures: Lack of Standard Nuclear Solution to the Primordial 7Li Problem" by M. Gai et al [PDF]

arXiv, 2020
The article recently published by M. Gai et al. claims to reach conclusions from a collaborative experiment dedicated to the study of the 7Be(n,alpha) reaction. These claims were published with no authorization from key collaborators, including a PI of the experiment. The Authors of the present Comment reject the conclusions of M. Gai et et al.
arxiv  

Inhibition of acyl‐CoA synthetase long‐chain isozymes decreases multiple myeloma cell proliferation and causes mitochondrial dysfunction

Molecular Oncology, EarlyView.
Triacsin C inhibition of the acyl‐CoA synthetase long chain (ACSL) family decreases multiple myeloma cell survival, proliferation, mitochondrial respiration, and membrane potential. Made with Biorender.com. Multiple myeloma (MM) is an incurable cancer of plasma cells with a 5‐year survival rate of 59%.
Connor S. Murphy, Heather Fairfield, Victoria E. DeMambro, Samaa Fadel, Carlos A. Gartner, Michelle Karam, Christian Potts, Princess Rodriguez, Ya‐Wei Qiang, Habib Hamidi, Xiangnan Guan, Calvin P. H. Vary, Michaela R. Reagan   +12 more
wiley   +1 more source

Response to neoadjuvant chemotherapy in early breast cancers is associated with epithelial–mesenchymal transition and tumor‐infiltrating lymphocytes

Molecular Oncology, EarlyView.
Epithelial–mesenchymal transition (EMT) and tumor‐infiltrating lymphocytes (TILs) are associated with early breast cancer response to neoadjuvant chemotherapy (NAC). This study evaluated EMT and TIL shifts, with immunofluorescence and RNA sequencing, at diagnosis and in residual tumors as potential biomarkers associated with treatment response.
Françoise Derouane, Jérôme Ambroise, Cédric van Marcke, Mieke Van Bockstal, Martine Berlière, Christine Galant, Hélène Dano, Médina Lougué, Elena Benidovskaya, Guy Jerusalem, Vincent Bours, Claire Josse, Jérôme Thiry, Aurélie Daumerie, Caroline Bouzin, Cyril Corbet, François P. Duhoux   +16 more
wiley   +1 more source

Molecular and functional profiling unravels targetable vulnerabilities in colorectal cancer

Molecular Oncology, EarlyView.
We used whole exome and RNA‐sequencing to profile divergent genomic and transcriptomic landscapes of microsatellite stable (MSS) and microsatellite instable (MSI) colorectal cancer. Alterations were classified using a computational score for integrative cancer variant annotation and prioritization.
Efstathios‐Iason Vlachavas, Konstantinos Voutetakis, Vivian Kosmidou, Spyridon Tsikalakis, Spyridon Roditis, Konstantinos Pateas, Ryangguk Kim, Kymberleigh Pagel, Stephan Wolf, Gregor Warsow, Antonia Dimitrakopoulou‐Strauss, Georgios N. Zografos, Alexander Pintzas, Johannes Betge, Olga Papadodima, Stefan Wiemann   +15 more
wiley   +1 more source

The Hidden Curriculum Challenges in Learning Professional Ethics Among Iranian Medical Students: A Qualitative Study

Advances in Medical Education and Practice, 2020
Yahya Safari,1 Alireza Khatony,2 Mohammad Rasoul Tohidnia3 1Research Center for Environmental Determinants of Health (RCEDH), Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran; 2Clinical Research Development Center, Imam Reza ...
Safari Y, Khatony A, Tohidnia MR
doaj  

Identification of phosphorylation site on PARP1 mediating its cytosolic translocation in virus-infected HeLa cells

STAR Protocols, 2022
Summary: Poly (ADP-ribose) polymerase 1 (PARP1) localization is controlled by its phosphorylation state. Here, we describe a protocol to monitor PARP1 subcellular localization in HSV-1-infected HeLa cells using immunofluorescence microscopy and ...
Fei Wang, Ming Tong Ma, Junfang Xu, Haipeng Liu   +3 more
doaj  

Languaging Ethics in Technology Practice [PDF]

arXiv, 2023
Ethics as embodied by technology practitioners resists simple definition, particularly as it relates to the interplay of identity, organizational, and professional complexity. In this paper we use the linguistic notion of languaging as an analytic lens to describe how technology and design practitioners negotiate their conception of ethics as they ...
arxiv  

Plasma lipidomic and metabolomic profiles in high‐grade glioma patients before and after 72‐h presurgery water‐only fasting

Molecular Oncology, EarlyView.
Presurgery 72‐h fasting in GB patients leads to adaptations of plasma lipids and polar metabolites. Fasting reduces lysophosphatidylcholines and increases free fatty acids, shifts triglycerides toward long‐chain TGs and increases branched‐chain amino acids, alpha aminobutyric acid, and uric acid.
Iris Divé, Lisa Hahnefeld, Katharina J. Wenger, Donat Kögel, Joachim Steinbach, Gerd Geisslinger, Michael W. Ronellenfitsch, Irmgard Tegeder   +7 more
wiley   +1 more source

Protocol to assess receptor-ligand binding in C. elegans using adapted thermal shift assays

STAR Protocols, 2023
Summary: The Caenorhabditis elegans genome encodes a greatly expanded number of nuclear hormone receptors, many of which remain orphaned. Here, we present a protocol to assess ligand-receptor binding in C.
Samantha Y. Tse, Read Pukkila-Worley
doaj