Results 61 to 70 of about 2,395,767 (232)

Trust, Justice, and Expertise in Nuclear Waste Management: A Q-Method Analysis of Environmental Discourses in the United Kingdom

open access: yesEthics, Policy & Environment
Nuclear waste is ethically contentious, concerning institutional trust, community engagement and the role nuclear plays in different sociotechnical configurations of energy futures.
Lee Towers, Matthew Cotton
semanticscholar   +1 more source

Energy options from the 20th Century: Comparing Conventional and Nuclear Energy from a Sustainable Standpoint

open access: yesJournal of Natural Resources and Development, 2012
Different Energy options have been the driving force for the world economy with an evolution in types and sources. Decades ago choosing what energy option to use did not call for much debate as issues of sustainability, pressure on our environment, and ...
Eric Ndeh Mboumien Ngang
doaj   +1 more source

Protocol for isolating nuclei from frozen fish heart tissue for single-cell genomic assays

open access: yesSTAR Protocols
Summary: Unbiased isolation of intact single nuclei from complex tissues, such as cryopreserved hearts, for massively parallel single-cell genomic assays is particularly challenging.
Chaoguang Wei   +5 more
doaj   +1 more source

Protocol for isolating cervico-vaginal fluid cells from Macaca mulatta to study immunological and functional changes during pregnancy

open access: yesSTAR Protocols
Summary: Cervico-vaginal fluid represents an important biological fluid due to its proximity to gestational tissues, such as the cervix, fetal membranes, and myometrium.
Daniel Short   +5 more
doaj   +1 more source

Protocol for the purification and analysis of nuclear UFMylated proteins

open access: yesSTAR Protocols
Summary: Protein UFMylation regulates numerous cellular processes including ribosome quality control and nuclear DNA repair. Here, we present a technique to isolate nuclei and purify UFMylated proteins under denaturing non-reducing conditions from ...
Pudchalaluck Panichnantakul   +1 more
doaj   +1 more source

An optimized protocol for the extraction and quantification of cytosolic DNA in mammalian cells

open access: yesSTAR Protocols
Summary: Leakage of mitochondrial or nuclear DNA into the cytosol can occur following viral infections, radiation damage, and some cancers. Here, we present an optimized protocol for isolating and quantifying cytosolic DNA from mammalian cells.
Aminu S. Jahun   +2 more
doaj   +1 more source

Protocol for 3D multiplexed fluorescent imaging of pulmonary TB lesions using Opal-TSA dyes for signal amplification

open access: yesSTAR Protocols
Summary: To detect multiple protein markers, here we present a protocol for imaging pulmonary tuberculosis (PTB) lesions from murine lungs using Opal-tyramide signal amplification (TSA) conjugated fluorescent dyes in free-floating formalin-fixed thick ...
Suruchi Lata   +6 more
doaj   +1 more source

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