Results 121 to 130 of about 455,210 (249)

Current status of multilayer neutron interferometry with gaseous samples at J-PARC. [PDF]

J Appl Crystallogr
Nambu T, Auton CJ, Fujiie T, Hino M, Hosobata T, Ichikawa G, Kitaguchi M, McElhannon AL, Mishima K, Seki Y, Shimizu HM, Snow WM, Yamagata Y.   +12 more
europepmc   +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Protocol to quantify glioblastoma cell invasion and nuclear deformations in 3D hydrogels. [PDF]

STAR Protoc
van Bodegraven EJ, Kesenci Y, Olivo-Marin JC, Boquet-Pujadas A, Etienne-Manneville S.   +4 more
europepmc   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Multicenter comparison of radionuclide calibrators and SPECT/CT protocols for quantitative ¹⁷⁷Lu   imaging in clinical practice. [PDF]

EJNMMI Phys
Claeys W, Baete K, Beels L, Bernard C, Danieli R, D'Asseler Y, De Crop A, Hesse M, Nuttens V, Vanderlinden B, Koole M.   +10 more
europepmc   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Integrative Taxonomy Reveals a Candidate Lineage Within the <i>Rhinolophus macrotis</i> Group. [PDF]

Biology (Basel)
Cong J, Zhang J, Yu H, Lei J, Miao G, Yang H, Li Q, Zhang Z, Csorba G, Sun K, Liu T.   +10 more
europepmc   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Multiplex immunofluorescence microscopy assays for pharmacodynamic assessment of MET tyrosine kinase activation in the plasma membrane and nucleus. [PDF]

PLoS One
Navas T, Srivastava AK, Lawrence SM, Hollingshead MG, Alcoser SY, Miller SB, Kinders RJ, Bottaro DP, Linehan WM, Kummar S, Doroshow JH, Parchment RE.   +11 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source