Results 321 to 330 of about 1,658,235 (358)
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Identity of nuclear membrane and non-histone nuclear proteins
Biochemistry, 1976The fate of plasma and nuclear membrane polypeptides in preparations of acidic chromosomal protein from chicken erythrocytes has been investigated. It is shown that detergent extraction procedures (Nonidet P-40, Triton X-100, and saponin), commonly employed in the preparation of acidic chromosomal protein, cannot be relied upon to remove plasma and ...
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Protein synthesis in nuclear residual protein
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 19651. The incorporation of radioactive amino acids into the nuclear residual protein from calf thymus has been investigated in vitro. 2. The nuclear residue has been found to incorporate actively [14C]tryptophan into its protein. 3. Optimal incorporation of [14C]tryptophan was obtained in the presence of Mg2+, adenosine 5′-triphosphate and an ...
Gordhan Patel, T. Y. Wang
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Nuclear Protein Transport Pathways
Nephron Experimental Nephrology, 1999Nuclear proteins like transcription factors and ribosomal proteins are synthesized in the cytoplasm and have to be transported into the nucleus to fulfill their functions. The transport of proteins >20–60 kD through the nuclear pore complex (NPC) into the nucleus is an active, energy-requiring process.
Enno Hartmann +2 more
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2007
Entry into the eukaryotic cell nucleus occurs through multiple pathways involving specific targeting signals, and intracellular receptor molecules of the importin/karyopherin superfamily which recognise and dock the nuclear import substrates carrying these signals at the nuclear pore.
Jade K. Forwood, David A. Jans
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Entry into the eukaryotic cell nucleus occurs through multiple pathways involving specific targeting signals, and intracellular receptor molecules of the importin/karyopherin superfamily which recognise and dock the nuclear import substrates carrying these signals at the nuclear pore.
Jade K. Forwood, David A. Jans
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Immunolocalization of Nuclear Proteins [PDF]
, 1994 Immunohistochemistry permits the localization at the tissue and subcellular level of antigens for which specific antisera or monoclonal antibodies are available. A wide variety of methods are used for this purpose, and the best method for any particular application must unfortunately be determined empirically.
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Pest sequences in nuclear proteins
International Journal of Biochemistry, 19931. Most of proteins which are rapidly degraded inside eukaryotic cells have been found to contain amino acid sequences (PEST sequences) enriched in proline, acidic residues (glutamic acid and/or aspartic acid) and hydrophilic residues (serine and threonine) (Rogers et al. (1986) Science 234, 364-368). 2.
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siRNA Screening of Nuclear Proteins
2014Functional gene screening is a basic strategy for identifying candidate genes that are responsible for biological processes of interest to researchers. RNA-mediated interference (RNAi)-based screening is one such approach. Although the knockdown technique using siRNA is widely accepted, careful evaluation is required to avoid false positive candidates ...
Yuko Hasegawa, Shinichi Nakagawa
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Nuclear protein tyrosine kinases
Trends in Biochemical Sciences, 1994Protein tyrosine phosphorylation plays an important role in the transduction of extracellular signals. The prototypical protein tyrosine kinases are localized at the plasma membrane and are coupled to receptors that bind extracellular factors. Thus, protein tyrosine phosphorylation was previously thought to occur only in the cytoplasm. However, several
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Nuclear shuttling: The default pathway for nuclear proteins?
Cell, 1993Ronald A. Laskey, C. Dingwall
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