Results 91 to 100 of about 6,528,274 (245)

Phosphomimetic mutations near active sites of proteins in Thermus thermophilus suggest a widespread regulatory mechanism

open access: yesFEBS Open Bio, EarlyView.
In Thermus thermophilus, more than half of the phosphorylation sites identified by proteomic analysis are located near the active site. All phosphomimetic mutants of phosphosites of six enzymes belonging to different families showed severely reduced activity compared with the wild‐type, particularly in the turnover number.
Anzu Nishiwaki   +3 more
wiley   +1 more source

Microbial profile of the appendix niche in acute appendicitis: a novel sampling approach

open access: yesFEBS Open Bio, EarlyView.
This study utilized a novel sampling method, ERAT (i.e. endoscopic retrograde appendicitis treatment)‐guided lumen aspiration, to obtain samples from the appendix, and shotgun metagenomic sequencing was performed for in situ characterization of the appendix microbiome in patients with acute appendicitis.
Huimin Ma   +10 more
wiley   +1 more source

Effect of compound nuclear reaction mechanism in 12C(Li6,d) reaction at sub-Coulomb energy

open access: yesPhysics Letters B, 2017
The angular distribution of the 12C(Li6,d) reaction populating the 6.92 and 7.12 MeV states of 16O at sub-Coulomb energy (E=cm3 MeV) are analysed in the framework of the Distorted Wave Born Approximation (DWBA).
Ashok Mondal, S. Adhikari, C. Basu
doaj   +1 more source

Structural and functional characterization of chitinase from carnivorous plant Drosera adelae

open access: yesFEBS Open Bio, EarlyView.
A class I chitinase from the carnivorous plant Drosera adelae was expressed and purified using a yeast system, revealing high enzymatic activity. Structural analyses of the catalytic and chitin‐binding domains identified key tyrosine residues involved in substrate binding, offering insights into the enzyme's adaptation for insect digestion.
Kazunari Yoneda   +7 more
wiley   +1 more source

Diffusion‐based size determination of solute particles: a method adapted for postsynaptic proteins

open access: yesFEBS Open Bio, EarlyView.
We present a diffusion‐based approach for measuring the size of macromolecules and their complexes, and demonstrate its use on postsynaptic proteins. The method requires fluorescein‐labelled protein samples, a microfluidic device that maintains laminar flow for said samples, a microscope recording the emitted fluorescent signals, and an analytic ...
András László Szabó   +7 more
wiley   +1 more source

Investigation of the interactions and electromagnetic shielding properties of graphene oxide/platinum nanoparticle composites prepared under low-dose gamma irradiation

open access: yesScientific Reports
A low-dose gamma irradiation was used for the one-step synthesis of graphene oxide/platinum nanoparticle composites. Various spectroscopic and microscopic methods were employed to structurally and morphologically characterize the prepared composites, and
Dejan Kepić   +11 more
doaj   +1 more source

Establishment and optimization of the two‐step induction system for generating primordial germ cell‐like cells from chicken embryonic stem cells

open access: yesFEBS Open Bio, EarlyView.
This study optimizes a two‐step induction system to generate PGCLCs from chicken ESCs. The induced cells express germline markers and migrate to gonads in ovo, offering a novel approach for avian genetic resource conservation. Primordial germ cells (PGCs) are the progenitor cells of sperm and eggs.
Zeyu Li   +10 more
wiley   +1 more source

Advances in nuclear reaction calculations by incorporating information from nuclear mean-field theories

open access: yesEPJ Web of Conferences, 2017
Mean-field model calculations for nuclear structure theories are combined with the statistical Hauser-Feshbach code in order to improve predictive capabilities of nuclear reaction for experimentally unknown cross sections.
Kawano Toshihiko
doaj   +1 more source

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