Results 161 to 170 of about 3,983,345 (293)

Development and Validation of a Duplex RT-qPCR for Detection of Peach Latent Mosaic Viroid and Comparison of Different Nucleic-Acid-Extraction Protocols. [PDF]

Plants (Basel), 2023
Luigi M, Taglienti A, Corrado CL, Cardoni M, Botti S, Bissani R, Casati P, Passera A, Miotti N, De Jonghe K, Everaert E, Olmos A, Ruiz-García AB, Faggioli F.   +13 more
europepmc   +1 more source

Dual‐Gene Edited Extracellular Vesicles Remodel the Redox Homeostasis to Inhibit Ferroptosis in Intervertebral Disc Degeneration

Advanced Science, EarlyView.
ABSTRACT Intervertebral disc degeneration (IDD) is driven by ferroptosis of nucleus pulposus cells (NPCs) as a core pathological mechanism. Nucleus pulposus progenitor cells (NPPCs), exhibiting stem cell‐like properties, yield extracellular vesicles (PEVs) with high affinity for NPCs and enable targeted phenotypic regulation.
Jing Yan, Yazhou Lin, Shuo Miao, Minghao Chao, Chengbai Dai, Ziwen Hao, Rui Chen, Ming Guan, Bin Pan, Fenglei Gao, Quan Zhou   +10 more
wiley   +1 more source

Optimizing Nucleic Acid Extraction from Extended Bovine Semen for Endemic and High-Consequence Pathogens. [PDF]

Animals (Basel)
Zimmerman A, Vandenburg-Carroll A, Marthaler DG, Lim A.   +3 more
europepmc   +1 more source

A Cascaded DNA Nanocircuit for Multi‐Signal‐Responsive Precision siRNA Delivery in Cancer Therapy

Advanced Science, EarlyView.
A cascaded dual‐AND logic DNA nanocircuit is engineered to respond to three tumor‐specific signals in a sequential manner: extracellular acidic pH, membrane‐overexpressed nucleolin (NCL), and intracellular glutathione (GSH). This programmable system selectively releases siPARP1 in glioblastoma (GBM), effectively silencing PARP1 and reversing TMZ ...
Yan Zhao, Yufei Lan, Min‐Goo Lee, Jinjun He, Yueran Pan, Eunji Kim, Xin Fu, Sungwook Jung, Wanxia Lu, Jinyue Fan, Haoxiang Chen, Yuanbo Zhao, Yuling Xu, Jong Seung Kim, Chao Zhang   +14 more
wiley   +1 more source

Variability analysis of a low-cost paper dipstick nucleic acid extraction method for wastewater surveillance using gage repeatability and reproducibility. [PDF]

Sci Rep
Ahuja S, Kulkarni A, Kondabagil K, Tallur S.   +3 more
europepmc   +1 more source

Digital PCR-based evaluation of nucleic acid extraction kit performance for the co-purification of cell-free DNA and RNA. [PDF]

Hum Genomics, 2022
Deleu J, Schoofs K, Decock A, Verniers K, Roelandt S, Denolf A, Verreth J, De Wilde B, Van Maerken T, De Preter K, Vandesompele J.   +10 more
europepmc   +1 more source

Artificial Intelligence‐Empowered Single‐Cell Phenotyping for Rapid, Automated Pathogen Diagnostics

Advanced Science, EarlyView.
This work presents an integrated diagnostic platform that combines microfluidic single‐cell bacterial detection with artificial intelligence‐driven analysis for rapid antimicrobial susceptibility testing. Single‐cell phenotyping enables assessment of antibiotic response in only a few cell replication cycles, while AI analysis supports precise bacterial
Sabita Khadka, Bushra Raahat, Spyros Tragoudas, Hui Li   +3 more
wiley   +1 more source

Correction: A rapid and high-yield method for nucleic acid extraction. [PDF]

Sci Rep
Vutukuru MR, Sharma DK, Chakraborty I, Mukhopadhyay D, Mitra N.   +4 more
europepmc   +1 more source

Defect‐Engineered BiO2‐X Nanosheets Mediate Sono‐Thermomechanical ECM Remodeling for Hepatocellular Carcinoma Immunotherapy

Advanced Science, EarlyView.
Herein, an ultrasound‐responsive BiO2‐X/siFn1 nanoplatform is developed to overcome anti‐PD‐1 resistance in hepatocellular carcinoma. Upon ultrasound irradiation, the platform downregulates fibronectin expression, while its sonothermal effect softens the dense extracellular matrix and activates antitumor immunity in the tumor microenvironment, thereby ...
Huimin Tian, Shen Zhang, Bolin Wu, Yichi Chen, Haitao Shang, Haoyan Tan, Chunyue Wang, Weichen Xu, Huixiong Xu, Haohao Yin, Wen Cheng   +10 more
wiley   +1 more source

Comparison of paper-based nucleic acid extraction materials for point-of-care testing applications. [PDF]

Cellulose (Lond), 2022
Tang R, Li M, Yan X, Xie M, Liu LN, Li Z, Xu F.   +6 more
europepmc   +1 more source