Results 221 to 230 of about 3,983,345 (293)

Neuron‐Derived Vesicular circMagi1 Restrains Vascular Repair After Ischemic Stroke by Promoting TRIM65‐Mediated ANXA2 Degradation

Advanced Science, EarlyView.
After ischemic stroke, injured neurons transfer circMagi1 to endothelial cells through extracellular vesicles. Within endothelial cells, circMagi1 binds ANXA2 and enhances its engagement with TRIM65, promoting ANXA2 ubiquitination and proteasomal degradation to constrain angiogenic repair.
Youkun Bi, Qun Liu, Baohua Zou, Wenyuan Wang, Xinlong Qiao, Lin Gao, Chaoxu Miao, Ningning Huang, Shuqing Tang, Rui Guo, Changqing Zeng, Ce Jia, Yuanchao Xue, Xuejun Guo, Jianjun Chen, Huiwen Wang, Guangju Ji   +16 more
wiley   +1 more source

Developing Highly Effective Nanoparticle mRNA Therapeutic for Pediatric Acute Respiratory Distress Syndrome

Advanced Science, EarlyView.
Sepsis‑induced pediatric acute respiratory distress syndrome suppresses FOXF1 in lung endothelial cells which causes life‐threatening lung damage. To counter this, researchers developed nanoparticles that specifically target these cells and deliver FOXF1 mRNA.
Zicheng Deng, Wen Gao, Jonathan Do, Danli Lu, Ying‐Wei Lan, Andreas Damianos, Gautam Verma, Ali Al Siraj, Isabella Lowry, Lance Peter, Nicholas E. Banovich, Fei Sun, Hua He, Tanya V. Kalin, Vladimir V. Kalinichenko   +14 more
wiley   +1 more source

Detaching Photosensitive Nanoparticles from Cells Prevents Them from Remaining in Cells after Photoporation

Advanced Science, EarlyView.
Phenylboronic acid‐functionalized gold nanoparticles reversibly attach to cell‐surface glycans, enhancing photoporation‐mediated intracellular delivery. After irradiation, ATP competitively disrupts the boronic ester interactions and removes cell‐associated nanoparticles, markedly reducing residual gold.
Tao Lu, Mina Nikolić, Baihao Huang, Deep Punj, Herlinde De Keersmaecker, Thibaut Van Acker, Wenjia Xie, Florian Vanlauwe, Phillip Blondeel, Liesl De Graeve, Félix Sauvage, Ranhua Xiong, Chaobo Huang, Frank Vanhaecke, Kevin Braeckmans, Stefaan C. De Smedt   +15 more
wiley   +1 more source

Sonogenetics Nanomedicine Activates the cGAS‐STING Pathway for Immunotherapy of Triple‐Negative Breast Cancer

Advanced Science, EarlyView.
A HER3‐targeted sonogenetic lipid nanoparticle platform is developed for triple‐negative breast cancer therapy. An AI‐designed miniprotein inhibits HER3 signaling, while TRPV4 and NFAT‐responsive IL‐15 plasmids enable ultrasound‐controlled calcium signaling and cytokine expression. The system induces Ca2+ overload, ROS generation, mitochondrial damage,
Junya Song, Yan Liu, Shifeng Zhao, Cheng Zhu, Hongchang Guo, Jin Chang, Jun Kang   +6 more
wiley   +1 more source

Smartphone‐Guided Visible Light Modulation of RSNOs for Antimicrobial Activity via Controlled Nitric Oxide Release

Advanced Science, EarlyView.
A light‐activated catheter insert combines antimicrobial blue light with nitric oxide release to generate complementary oxidative and nitrosative stress, producing potent broad‐spectrum antimicrobial activity. The approach achieves greater than 4‐log reductions in bacterial and fungal viability and significantly reduces Staphylococcus aureus ...
Alexander Bruckmann, Adam Brooks Goodman, Myddelton C. Parker, Mark Garren, Ji Eun Park, Chad Schmiedt, Hitesh Handa, Elizabeth J. Brisbois   +7 more
wiley   +1 more source

Integrated device for plasma separation and nucleic acid extraction from whole blood toward point-of-care detection of bloodborne pathogens.

Lab Chip
Ayers AG, Victoriano CM, Sia SK.
europepmc   +1 more source

Concentrating Nucleic Acids by Extraction with Butanol

Cold Spring Harbor Protocols, 2017
During extraction of aqueous solutions with solvents such as secondary butyl alcohol (isobutanol) or n-butyl alcohol (n-butanol), a proportion of the water molecules is partitioned into the organic phase. Nucleic acids remain in the aqueous phase.
Michael R, Green, Joseph, Sambrook
openaire   +3 more sources

Some of the next articles are maybe not open access.

Related searches:

Extraction of Viral Nucleic Acids: Comparison of Five Automated Nucleic Acid Extraction Platforms

Journal of Clinical Virology, 2012
Nucleic acid extraction has a major impact on the reliability of results in routine molecular diagnostics. Optimal isolation of nucleic acids and removal of inhibitors are essential.This study compares five different automated extraction platforms for the extraction of norovirus RNA from stool and cytomegalovirus (CMV) DNA from plasma samples.Norovirus
Jens Verheyen, Rolf Kaiser, Harald H Kessler   +2 more
exaly   +3 more sources

Low-Resource Nucleic Acid Extraction Method Enabled by High-Gradient Magnetic Separation

ACS Applied Materials & Interfaces, 2020
Nucleic acid-based diagnostic tests often require isolation and concentration of nucleic acids from biological samples. Commercial purification kits are difficult to use in low-resource settings because of their cost and insufficient laboratory ...
Nicholas Adams, Stephanie I Pearlman, Frederick Russell Haselton   +2 more
exaly   +4 more sources

Extraction of Nucleic Acids

2006
The utility of DNA-or RNA-based testing depends, in large part, on the quality and nature of the diagnostic sample. A variety of methods is available to extract nucleic acids for analysis. The choice of technique should consider both the sample source and the nature of the eventual assay.
Paul N. Bogner, Anthony A. Killeen
openaire   +1 more source