Results 251 to 260 of about 119,828 (299)
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Identification of foodborne pathogens by nucleic acid hybridization
International Journal of Food Microbiology, 1991Nucleic acid hybridization methods have been developed and used to identify microorganisms in foods. Tests performed on mixed cultures save the time required to establish pure cultures. Enterotoxigenic or invasive strains of foodborne bacterial pathogens are detected with probes that identify genes responsible for virulence.
W E, Hill, S P, Keasler
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Nucleic acid hybridization in viral Hepatitis research
La Ricerca in Clinica e in Laboratorio, 1988Nucleic acid hybridization is extremely important in viral hepatitis research and the newly emerging techniques are now becoming an indispensable resource also in the diagnostic laboratory. This paper provides information on methods currently used for detection of viral nucleic acids with special emphasis on the importance of hepatitis B virus DNA in ...
Maran E +4 more
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Nucleic acid hybridization in viral diagnosis
Clinical Biochemistry, 1990Since 1982, numerous studies have been published utilizing a variety of hybridization techniques to detect viral nucleic acid directly in clinical specimens and in tissue sections. However, hybridization techniques are still not widely used in the clinical laboratory.
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New Developments in Nucleic Acid Hybridization
1985Nucleic acid hybridization is widely used for scientific applications in specialized laboratories. This paper describes hybridization probes that can be prepared with less specialized equipment. A new indirect 'sandwich' hybridization test is described which allows the use of only one universally usable labelled probe for hybridization tests with ...
H, Wolf +9 more
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Nucleic Acid Hybridization: Triplex Stability and Energetics
Annual Review of Biophysics and Biomolecular Structure, 1995In this chapter, we review the current state of the thermodynamic database for triple helical oligonucleotide hybridization reactions and present a critical assessment of the methods used to obtain the relevant data. The thermodynamic stability of triple-helix oligonucleotide constructs is discussed in terms of its dependence on temperature, chain ...
G E, Plum +3 more
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HYBRIDIZATION AND RENATURATION KINETICS OF NUCLEIC ACIDS
Annual Review of Biophysics and Bioengineering, 1976When two DNA or RNA molecules contain sequences of nucleotides that are complementary to one another, they are able to come together to form a double helix with all of the bases paired. If both strands are DNA, the process is usually called renaturation or reassociation; if one strand is DNA and the other is RNA, the process is called hybridization ...
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Nucleic Acid Hybridization in the Diagnosis of Viral Infections
Clinics in Laboratory Medicine, 1985Recombinant DNA technology, including molecular cloning and nucleic acid hybridization, is now being applied to problems in clinical virology. Although viral isolation in cell culture remains the most sensitive and specific diagnostic test for many viruses, for some viruses, isolation in cell culture is lengthy or difficult or has not yet been achieved.
M L, Landry, C K, Fong
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Hybridization of Nucleic Acids
1969I am not sure there is a true difference in DNA-DNA hybridization between DNA from young and old animals. This is a complicated question because of difficulties in preparing DNA completely free of protein, the great complexity of mammalian DNA compared to bacterial DNA, and the greater probability for non specific inter an intra molecular interaction ...
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Use of bioluminescence in nucleic acid hybridization reactions
Journal of Bioluminescence and Chemiluminescence, 1989AbstractLuminescence reactions can be used to detect specific nucleic acid sequences hybridized with a nucleic probe. Different labels such as cytidine sulphone, fluorescein, and biotin can be incorporated into DNA or oligonucleotide molecules and detected by antibody or avidin conjugates coupled to glucose‐6P dehydrogenase.On supports such as ...
P, Balaguer +3 more
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Microfluidic chip for fast nucleic acid hybridization
Lab on a Chip, 2003The design and experimental verification of a fast nucleic acid hybridization microchip using the fluidic velocity and strain rate effects was conducted. This hybridization chip was able to increase the hybridization signal 6-fold, reduce non-specific target-probe binding and background noise within 30 min, as compared to conventional hybridization ...
Yung-Chiang, Chung +3 more
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