Results 81 to 90 of about 471,867 (378)

Identification of a novel methyltransferase, Bmt2, responsible for the N-1-methyl-adenosine base modification of 25S rRNA in "Saccharomyces cerevisiae" [PDF]

, 2013
The 25S rRNA of yeast contains several base modifications in the functionally important regions. The enzymes responsible for most of these base modifications remained unknown.
Entian, Karl-Dieter, Kötter, Peter, Sharma, Sunny, Watzinger, Peter   +3 more
core   +1 more source

Nanodiamond Quantum Sensors for Probing Free Radical Biology

Advanced Functional Materials, EarlyView.
Free radicals play key roles in cellular signaling and disease but remain difficult to measure in living systems. Nanodiamonds (NDs) with nitrogen‐vacancy (NV) centers enable quantum sensing of local magnetic noise via T₁ relaxometry, providing nondestructive radical detection in living cells.
Qi Lu, Yingke Wu, Tanja Weil
wiley   +1 more source

Label-free, background-free detection of nucleic acid with immobilization-free heterogeneous biosensor and one-pot hybridization chain reaction amplification

Advanced Sensor and Energy Materials
Although immobilization-free and label-free electrochemical DNA (E-DNA) biosensors have engaged tremendous interest due to their superior properties, such as easy operation, time-saving and cost-saving, most of them are fabricated in homogeneous modes ...
Hongyan Yang, Zeyu Ma, Dan Zhang, Yi Wang, Lei Li, Guobao Zhou   +5 more
doaj   +1 more source

Cooperative Oligonucleotides in Purification of Cycle Sequencing Products

BioTechniques, 2000
Nucleic acid hybridization is an essential component in many of today's standard molecular biology techniques. In a recent study, we investigated whether nucleic acid capture could be improved by taking advantage of stacking hybridization, which refers ...
A. Blomstergren, D. O'Meara, M. Lukacs, M. Uhlén, J. Lundeberg   +4 more
doaj   +1 more source

Method of identifying inhibitors of glutathione S-transferase (GST) gene expression [PDF]

, 1999
Complementary DNA and genomic clones for three variants of GST-.pi. are disclosed. It is demonstrated that certain of these variants are overexpressed in gliomas, thereby indicating an involvement with that form of cancer.
Akande, Olanike, Ali-Osman, Francis, Antoun, Gamil, Buolamwini, John K., Keller, Charles W., Lo, Hui-Wen, Lopez-Berestein, Gabriel   +6 more
core   +1 more source

Targeting Post‐Irradiation Thyroid Dysfunction: Electrospun Scaffolds As A Dual‐Action Approach for Antioxidant and Immune Modulation

Advanced Healthcare Materials, EarlyView.
Radiation‐induced hypothyroidism follows head and neck radiotherapy due to oxidative stress and inflammation. Electrospun polycaprolactone scaffolds containing adenosine have potential to modulate thyroid repair. Scaffolds enhance thyrocyte proliferation, antioxidant enzymes glutathione peroxidase and catalase, reduce senescence and apoptosis markers ...
Maria Heim, Ella‐Louise Handley, Daniel Grant, Lizi M. Hegarty, Elaine Emmerson, Anthony Callanan   +5 more
wiley   +1 more source

Diagnosis of plant viruses by nucleic acid hybridization

Agricultural and Food Science, 1987
Nucleic acid hybridization is a powerful technique for the diagnosis of many plant viruses not easily detected by serological techniques. It is particularly effective in the detection of viruses occurring in low amount in plant tissue, viruses that are ...
Reijo Karjalainen, Leo Rouhiainen, Hans Söderlund   +2 more
doaj  

Capturing the ‘ome’ : the expanding molecular toolbox for RNA and DNA library construction [PDF]

, 2018
All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences.
Boone, Morgane, Callewaert, Nico, De Koker, Andries   +2 more
core   +2 more sources

Engineering Extracellular Vesicle Production Through Magnetic Ion Channel Activation for Bone Regeneration

Advanced Healthcare Materials, EarlyView.
Magnetic Ion Channel Activation (MICA) enables remote stimulation of mechanosensitive ion channels using functionalised magnetic nanoparticles, enhancing extracellular vesicle (EV) biogenesis is pre‐osteoblasts. MICA desrived EVs exhibit typical nano‐vesicular characteristics but display superior pro‐osteogeneic activity, promoting mesenchymal stem ...
Afeesh Rajan Unnithan, Kenny Man, Kritika, Lee A. Gethings, Christopher J. Hughes, Alicia Keenan, Liam Heaney, Sophie C. Cox, Owen G. Davies, Alicia J. El Haj   +9 more
wiley   +1 more source

Molecular biologic techniques in ophthalmic pathology

Indian Journal of Ophthalmology, 1998
The polymerase chain reaction (PCR) and nucleic acid hybridization assays are recently introduced molecular techniques that allow for the identification of extremely small quantities of specific nucleic acids. These techniques have significant advantages
Rajeev Buddi, Biswas Jyotirmay
doaj