Results 41 to 50 of about 107,694 (265)
FEBS Letters, EarlyView.We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee +3 more
wiley +1 more source
Ciência e Agrotecnologia, 2008 Resultados experimentais seguros são obtidos por meio da utilização de adequados tamanhos de amostras e número de repetições. O conhecimento do melhor tamanho de amostra tem relevada importância, quando se deseja reduzir os custos na pesquisa ...
Thiago Rodrigues Catapatti +3 more
doaj +1 more source
FEBS Letters, EarlyView.We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley +1 more source
Tau acetylation at K331 has limited impact on tau pathology in vivo
FEBS Letters, EarlyView.We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto +3 more
wiley +1 more source
Ciência Rural, 2005 O uso de bordaduras, aliado ao número adequado de repetições para definir um plano experimental, tem sido pouco estudado. Dois híbridos de milho foram avaliados com o objetivo de estimar o número de repetições necessários, substituindo-se o uso de ...
Sérgio José Ribeiro de Oliveira +5 more
doaj +1 more source
Replication timing is regulated by the number of MCMs loaded at origins
Genome Research, 2015 Replication timing is a crucial aspect of genome regulation that is strongly correlated with chromatin structure, gene expression, DNA repair, and genome evolution. Replication timing is determined by the timing of replication origin firing, which involves activation of MCM helicase complexes loaded at replication origins.
Das, Shankar P. +5 more
openaire +2 more sources
Plot size and number of replications in Sudan grass
Semina: Ciências Agrárias, 2020 The aim of this study was to determine the optimal plot size and the number of replications to evaluate fresh weight in Sudan grass [Sorghum sudanense (Piper) Stapf.]. Twenty-six uniformity trials were carried out in two cultivars (BRS Estribo and CG Farrapo), in four sowing seasons (20 Dec, 20 Jan, 7 Feb and 24 Feb) and two methods for evaluating ...
Alberto Cargnelutti Filho +7 more
openaire +2 more sources
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
FEBS Letters, EarlyView.Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura +2 more
wiley +1 more source
Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation
FEBS Letters, EarlyView.Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz +11 more
wiley +1 more source