Results 171 to 180 of about 15,530,041 (302)

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

FEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source

Differential Recognition of Lipopolysaccharide O-Antigens by the Pattern Recognition Molecules Mannan-Binding Lectin and Ficolins of the Complement System. [PDF]

J Innate Immun
Hymøller KM, Crone L, Thiel S, Hennet T.   +3 more
europepmc   +1 more source

In vitro generation of regulatory T cells: A challenging tool for studying immune aging in humans

FEBS Open Bio, EarlyView.
In vitro induction of human regulatory T cells using standard protocols generates suppressive, Foxp3‐expressing CD4+ T cells independently of TGF‐β supplementation and donor age. Regulatory T (Treg) cells play a key role in immune tolerance and homeostasis.
Lynn Muller, Jana Bleher, Birgit Weinberger, Lourdes Rocamora‐Reverte   +3 more
wiley   +1 more source

MALDI glycotyping of O-antigens from a single colony of gram-negative bacteria. [PDF]

Sci Rep
Urakami S, Hinou H.
europepmc   +1 more source

Identifying genetic diversity of O antigens in Aeromonas hydrophila for molecular serotype detection. [PDF]

PLoS One, 2018
Cao H, Wang M, Wang Q, Xu T, Du Y, Li H, Qian C, Yin Z, Wang L, Wei Y, Wu P, Guo X, Yang B, Liu B.   +13 more
europepmc   +1 more source

Ultrafast visual detection of Streptococcus suis via HtpsC‐targeted LAMP–CRISPR–Cas12a coupled with lateral flow immunochromatography

FEBS Open Bio, EarlyView.
A rapid visual test combines DNA amplification, CRISPR–based target verification, and a lateral flow strip to detect Streptococcus suis. The assay gives results in about 45 min, reduces false‐positive interpretation, and does not require fluorescence equipment.
Liu‐xin Li, Miao Xin, Xiao‐wen Cui, Shi‐yin Yu, Fei Meng, Min Cao, Xiao‐hui Xiong, Yi‐chen Lu   +7 more
wiley   +1 more source

Genetic diversity of the O antigens of Proteus species and the development of a suspension array for molecular serotyping. [PDF]

PLoS One, 2017
Yu X, Torzewska A, Zhang X, Yin Z, Drzewiecka D, Cao H, Liu B, Knirel YA, Rozalski A, Rozalski A, Wang L.   +10 more
europepmc   +1 more source

Five‐Year Outcomes of Laparoscopic Sphincter‐Preserving Surgery for Rectal Cancer: A Prospective Study in Elderly Vietnamese Patients

Aging and Cancer, EarlyView.
This prospective study demonstrates that laparoscopic sphincter‐preserving surgery is feasible for elderly patients. While overall survival reaches 70% at 5 years, advanced T‐stage and the omission of neoadjuvant therapy significantly drive recurrence, highlighting the need for personalized geriatric protocols despite logistical challenges.
Huu Duc Ho, Quang Le, Ngoc Thach Tran, Van Viet Ung, Loc Vu   +4 more
wiley   +1 more source

Deciphering minimal antigenic epitopes associated with Burkholderia pseudomallei and Burkholderia mallei lipopolysaccharide O-antigens. [PDF]

Nat Commun, 2017
Tamigney Kenfack M, Mazur M, Nualnoi T, Shaffer TL, Ngassimou A, Blériot Y, Marrot J, Marchetti R, Sintiprungrat K, Chantratita N, Silipo A, Molinaro A, AuCoin DP, Burtnick MN, Brett PJ, Gauthier C.   +15 more
europepmc   +1 more source

Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level

Aging and Cancer, EarlyView.
This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley   +1 more source