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Identification of Salmonella O antigens by coagglutination
Journal of Clinical Microbiology, 1984This study concerns the preparation of reagents for identifying the somatic O antigens of Salmonella enteritidis. Coagglutination reagents (COAGs) with antibody fixed to killed and stabilized protein A-bearing staphylococci were prepared with antisera which were used for identifying the somatic O antigens of S.
J O, Cohen, L E, Britt, W K, Harrell
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Immunochemistry of Salmonella O-Antigens
International Archives of Allergy and Applied Immunology, 2009The binding specificities of antibodies directed against the Salmonella sero-group A-specific O-antigen 2 determinant were characterized by precipitation-inhibition and enzyme-linked immunosorbent assay inhibition tests. Two different antigen O-2-specific antisera were investigated: one conventional factor O-2-serum (elicited by whole heat-killed ...
H. Jörbeck, S.B. Svenson, A.A. Lindberg
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The O-antigen of Salmonella enterica O13 and its relation to the O-antigen of Escherichia coli O127
Carbohydrate Research, 2010The following structure of the O-polysaccharide (O-antigen) of Salmonella enterica O13 was established by chemical analyses along with 2D (1)H and (13)C NMR spectroscopy: -->2)-alpha-l-Fucp-(1-->2)-beta-d-Galp-(1-->3)-alpha-d-GalpNAc-(1-->3)-alpha-d-GlcpNAc-(1--> The O-antigen of S.
Andrei V, Perepelov +7 more
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Structural studies of the Vibrio cholerae o-antigen
Carbohydrate Research, 1979The dominant part of the O-antigen of Vibrio cholerae is a homopolysaccharide composed of (1 leads to 2)-linked 4-amino-4,6-dideoxy-alpha-D-mannopyranosyl (perosaminyl) residues, in amino groups of which are acylated by 3-deoxy-L-glycero-tetronic acid. Most of the amino sugar is decomposed during acid hydrolysis. Treatment of the polymer with anhydrous
L, Kenne +4 more
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Biosynthesis of lipopolysaccharide O antigens
Trends in Microbiology, 1995Lipopolysaccharide O antigens are important virulence determinants for many bacteria. O-antigen synthesis is an interesting problem in cell-surface assembly. There are two known assembly pathways, which differ in the cellular location of their polymerization steps and in the direction of chain polymerization.
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In Vitro O-Antigen Ligase Assay
2013WaaL is a membrane enzyme that catalyzes the glycosidic bonding of a sugar at the proximal end of the undecaprenyl-diphosphate (Und-PP)-O-antigen with a terminal sugar of the lipid A-core oligosaccharide (OS). This is a critical step in lipopolysaccharide synthesis.
Ruan, Xiang, Valvano, Miguel A
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2011
The lipopolysaccharide (LPS) is the major constituent of the outer leaflet of the outer membrane of Gram-negative bacteria. Its lipid A moiety is embedded in the membrane and serves as an anchor for the rest of the LPS molecule. The outermost repetitive glycan region of the LPS is linked to the lipid A through a core oligosaccharide (OS), and is ...
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The lipopolysaccharide (LPS) is the major constituent of the outer leaflet of the outer membrane of Gram-negative bacteria. Its lipid A moiety is embedded in the membrane and serves as an anchor for the rest of the LPS molecule. The outermost repetitive glycan region of the LPS is linked to the lipid A through a core oligosaccharide (OS), and is ...
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Carbohydrate Research, 2010
O-Polysaccharides (O-antigens) were isolated from Escherichia coli O13, O129, and O135 and studied by chemical analyses along with 2D (1)H and (13)C NMR spectroscopy. They were found to possess a common -->2)-l-Rha-(alpha1-->2)-l-Rha-(alpha1-->3)-l-Rha-(alpha1-->3)-d-GlcNAc-(beta1--> backbone, which is a characteristic structural motif of the O ...
Andrei V, Perepelov +7 more
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O-Polysaccharides (O-antigens) were isolated from Escherichia coli O13, O129, and O135 and studied by chemical analyses along with 2D (1)H and (13)C NMR spectroscopy. They were found to possess a common -->2)-l-Rha-(alpha1-->2)-l-Rha-(alpha1-->3)-l-Rha-(alpha1-->3)-d-GlcNAc-(beta1--> backbone, which is a characteristic structural motif of the O ...
Andrei V, Perepelov +7 more
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Carbohydrate Research, 2003
The O-antigen is an important component of the outer membrane of Gram-negative bacteria. It is a repeat unit polysaccharide and consists of a number of repeats of an oligosaccharide, the O-unit, which generally has between two and six sugar residues.
Gabrielle, Samuel, Peter, Reeves
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The O-antigen is an important component of the outer membrane of Gram-negative bacteria. It is a repeat unit polysaccharide and consists of a number of repeats of an oligosaccharide, the O-unit, which generally has between two and six sugar residues.
Gabrielle, Samuel, Peter, Reeves
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Detection of O antigens inEscherichia coli
Animal Health Research Reviews, 2011AbstractLipopolysaccharide on the surface ofEscherichia coliconstitutes the O antigens which are important virulence factors that are targets of both the innate and adaptive immune systems and play a major role in host–pathogen interactions. O antigens are responsible for antigenic specificity of the strain and determine the O serogroup.
Chitrita, DebRoy +2 more
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