Results 131 to 140 of about 497,699 (317)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Validity and Agreement of Uveitis Experts in Interpretation of Ocular Photographs for Diagnosis of Behcet Uveitis

open access: yes, 2014
Purpose: To evaluate agreement and estimate sensitivity and specificity of uveitis specialists' interpretation of ocular photographs in diagnosing Behcet uveitis.
AKMAN, MEHMET
core   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Ocular pathology /

open access: yes
Comprehensive, yet concise and clinically oriented, the new edition of Ocular Pathology brings you the very latest advances of every aspect of ocular pathology. From updated information on today's imaging techniques, to the implementation of genetic data
Sassani, Joseph W.,author.   +1 more
core  

Automedicação tópica ocular em Florianópolis - Santa Catarina. [PDF]

open access: yes, 1998
Trabalho de Conclusão de Curso - Universidade Federal de Santa Catarina, Centro de Ciências da Saúde, Departamento de Clínica Médica, Curso de Medicina, Florianópolis ...
Müller, Simone
core  

Hyphema as a Complication of Blunt Ocular Trauma and Additional Ocular Findings

open access: yes, 2014
Objectives: To investigate the frequency of angle recession, commotio retinae, and other ocular findings in patients with hyphema due to blunt ocular trauma.
Seda Adıyeke   +4 more
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Chronic inflammation as a proposed risk factor for ocular surface squamous neoplasia

open access: yes
Chronic inflammation is a predisposing factor for metaplastic changes and ultimately dysplasia. We describe cases of OSSN occurring in the setting of chronic ocular surface inflammation.
Theotoka, Despoina   +7 more
core   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

open access: yesFEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke   +4 more
wiley   +1 more source

Review and analysis of grading scales for ocular surface staining

open access: yes, 2019
Vital dye staining has been used for over a century to assess the severity of ocular surface disease. However, despite common usage, a universally accepted “gold standard” grading scale does not exist for corneal and conjunctival staining, which can ...
Simpson, T   +5 more
core   +1 more source

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