Results 241 to 250 of about 1,639,925 (354)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

The Current Toolbox for Covalent Inhibitors: From Hit Identification to Drug Discovery. [PDF]

JACS Au
You M, Liu H, Li C.
europepmc   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

Beyond phase boundaries: atomic mechanisms governing structure and property variations in (K, Na)NbO₃-based ferroelectrics. [PDF]

Nat Commun
Lv X, Wang X, Shi X, Kong J, Huang HB, Hu T, Fu Z, Lyu J, Ma Y, Zhang XX, Wu B, Pramanick A, Wu J.   +12 more
europepmc   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

BioFuse: A programmable timer switch of gene expression. [PDF]

Sci Adv
Huang C, Zhao J, Tan Z, Dai S, Wang B, Xie Z, Zhang F, Zhou Y, Yu N, Cai C, Yao Y, Wang B, Tian B.   +12 more
europepmc   +1 more source

Patatin‐domain‐containing (phospho)lipases under control: Mammalian co‐regulators and pathogenic activation mechanisms

FEBS Open Bio, EarlyView.
Patatin domain‐containing (phospho)lipases are lipid‐hydrolyzing enzymes central to metabolism, membrane remodeling, and signaling. Their activity relies on precise co‐activation mechanisms involving protein–protein interactions and conformational rearrangements.
Noopur Dubey, Lina Riegler‐Berket, Monika Oberer   +2 more
wiley   +1 more source

Protein-nucleic acid language model-assisted design of precise and compact adenine base editor. [PDF]

Nat Commun
Ren J, Yao J, Cao Q, Li Y, Li Y, Zhang Z, Ge X, Wang S, Zhang Y, Wang X, Zhang X.   +10 more
europepmc   +1 more source

Performance Evaluation Model to Enhance Life Cycle Productivity in Modular and Off-Site Construction: A Process-Oriented Approach [PDF]

Babak Manouchehri, SangHyeok Han, Fuzhan Nasiri   +2 more
openalex   +1 more source