Results 71 to 80 of about 126,493 (296)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

KEBIJAKAN PEMERINTAH KOTA SURABAYA DALAM MEMANFAATKAN RUANG TERBUKA HIJAU KEBUN BIBIT BRATANG

Jurnal Review Politik, 2015
This study examines the policies of the Surabaya government in developing green-open spaces in the Bratang nurseries. Exploiting such green-open spaces becomes an answer to environmental problems, such as air and water pollution, faced by many urban ...
Nurul Hidayati
doaj   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Les espaces verts de la ville d’Assise

Projets de Paysage, 2008
This paper focuses on the management of green open spaces of Assisi town. Since 2000 the influx of more than one million tourists each year and the inscription at “World Heritage List” have played a role in the way of considering and managing open spaces.
Giulia Giacchè
doaj   +1 more source

Open Spaces, Green Areas: The Szeged Circular Embankment as a Green Ring in the Plans of Endre Pálfy-Budinszky

In 1879, the Hungarian city of Szeged was destroyed by the flooding Tisza River, necessitating its rebuilding from Lajos Lechner’s plans. With it, Szeged developed a central urban structure, with a circular embankment aligned with the boulevards ...
Anna Váraljai
core   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

THE THEORY OF SPACE CONCEPTS RELATED TO FORMULA GREEN OPEN SPACE

Journal of architecture&ENVIRONMENT, 2014
The concept of green open space is still stuck on the concept of space-based theory of absolute space by Issac Newton, who see space as a separation between the mass of the building as a container (solid) and open space (void). Meanwhile, the demands of the world's cities today to combine city life with the natural environment in the city increasingly ...
openaire   +2 more sources

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Investigating spatial and temporal characteristics and elements influencing running among residents of Nanchang city in green open areas

Computational Urban Science
Understanding the exercise behaviors of urban residents in green open spaces is crucial for optimizing future urban spatial planning and improving residents’ quality of life. This study provides an in-depth analysis of residents’ running behaviors across
Tianwei Fang, Linguo Zhou, Zhenrao Cai, Zhijin Tan, Chao Chen, Jiali Zheng, Chaoyang Fang   +6 more
doaj   +1 more source