Results 171 to 180 of about 141,855 (294)
, 2019 The physical texture of cities is comprised of built-up spaces, open-green spaces, and transportation arteries. Urbanization is a dynamic that compose of each of the factors above in a fair proportion to human beings and nature. On the other hand, due to
Aklıbaşında, Meliha +1 more
core
Evolution‐guided yeast complementation reveals functional differences in human PSPH variants
FEBS Open Bio, EarlyView.Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez +6 more
wiley +1 more source
Impact of environmental factors on cardiovascular health in Castilla-La Mancha: the CarVasCLiMa cohort study protocol. [PDF]
BMJ OpenSaz-Lara A +19 more
europepmc +1 more source
FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
FEBS Open Bio, EarlyView.Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Disaster-resilient cities and open green spaces
The rapid and unplanned urbanization processes worldwide intensify the adverse impacts of physical, social, and environmental factors, rendering cities more vulnerable. Balancing the numerous economic, environmental, administrative, and social elements within cities and reducing their vulnerability to become resilient against stress and shocks have ...
Demiroğlu, Demet +3 more
openaire +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Associations between natural area availability and morbidity: a cross-sectional study using health insurance billing data from Germany. [PDF]
BMJ OpenMüller D +6 more
europepmc +1 more source
Analysis of The Availability of Urban Green Open Space
Proceedings of the 3rd International Seminar on Tourism (ISOT 2018), 2019 Mamat Ruhimat, Riyan Zainudin
openaire +1 more source