Results 151 to 160 of about 126,493 (296)
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
The Standard of Parks and Green Open Spaces in China [PDF]
中国では文化大革命の時期において多くの都市公園が破壊された。改革開放後,経済の急速な発展により,都市に人口が集中した。そのため,都市環境問題の解決は,都市発展において重要な課題となり,公園緑地に関する施策が次々と策定された。本研究は中国の公園緑地に関わる法律,条例及び標準等を把握することにより都市計画,公園計画・設計,緑地分類などにおける公園分類など公園緑地標準について明らかにする。そして,都市緑地計画学の観点から,中国における公園緑地の位置づけと公園種別の特色を分析し ...
カネコ, タダカズ +13 more
core +1 more source
Production of space in the green open space at Universitas Mercu Buana
IOP Conference Series: Materials Science and Engineering, 2018 S.P. Tunjung Atmadi, Ali Ramadhan
openaire +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis
FEBS Open Bio, EarlyView.Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho +4 more
wiley +1 more source
Green spaces for heat-resilient neighbourhoods in Govan: Community research report [PDF]
From summer 2023 to the end of 2024, a community-led research project investigated green and natural spaces in Govan and their role in helping residents to cope with hotter weather.
Mabon, Leslie +1 more
core
YIPFα1A expression is regulated by multilayered molecular mechanisms
FEBS Open Bio, EarlyView.YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji +2 more
wiley +1 more source
Assessing the restorative benefits of open rooftop green spaces characteristics using virtual reality and EEG analysis. [PDF]
Front PsycholChen Z +6 more
europepmc +1 more source
FEBS Open Bio, EarlyView.The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer +7 more
wiley +1 more source