Results 181 to 190 of about 136,516 (290)

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

How urban green spaces influence adolescent mental health in urban fringe areas: A cultural ecosystem services perspective. [PDF]

iScience
Long Y, Zhen C, Cheng L, Kang F, Lin Y, Li J, Chen Z, Chen W, Liu X, Song Y.   +9 more
europepmc   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Analysis of The Availability of Urban Green Open Space

Proceedings of the 3rd International Seminar on Tourism (ISOT 2018), 2019
Mamat Ruhimat, Riyan Zainudin
openaire   +1 more source

Trends in greenspace use, socioeconomic inequalities and mental health benefits following COVID-19 from 2020 to 2025: a four-wave repeat cross-sectional study of UK adults. [PDF]

BMJ Public Health
Olsen JR, Nicholls N, Burnett H, Johnstone A, Mitchell R.   +4 more
europepmc   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Socioeconomic inequalities in exposure to neighbourhood environments for physical activity: a systematic review. [PDF]

Int J Behav Nutr Phys Act
van de Geest JDS, Cuenca VC, Schoonmade LJ, Cereijo L, Mora DJ, Meijer P, Lakerveld J.   +6 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Learning from real-world conditions to advance green care for mental health equity in the Global North: protocol for a transdisciplinary multi-scalar project. [PDF]

BMJ Open
Cole HVS, Caputo S, Specht K, Giedych R, Otsuka N, Cook PA, Gawryszewska B, Luciano JV, Olszewska-Guizzo A, Hedblom M, Houston-Read R, McVay K, Neuberger K, Sienkiewicz-Jarosz H, Feliu-Soler A, de Prado-Bert P, Hardman M, Howarth ML, Bragg R, Crichigno A, Triguero-Mas M.   +20 more
europepmc   +1 more source