Results 11 to 20 of about 1,366,220 (285)

Visualisation of dCas9 target search in vivo using an open-microscopy framework [PDF]

Nature Communications, 2019
Single-particle tracking PALM (sptPALM) provides quantitative information in vivo if the protein of interest remains in a single diffusional state during track acquisition.
Koen J. A. Martens, Sam P. B. van Beljouw, Simon van der Els, Jochem N. A. Vink, Sander Baas, George A. Vogelaar, Stan J. J. Brouns, Peter van Baarlen, Michiel Kleerebezem, Johannes Hohlbein   +9 more
doaj   +2 more sources

Open-3DSIM: an open-source three-dimensional structured illumination microscopy reconstruction platform

Nature Methods, 2023
Abstract Open-3DSIM is an open-source reconstruction platform for three-dimensional structured illumination microscopy. We demonstrate its superior performance for artifact suppression and high-fidelity reconstruction relative to other algorithms on various specimens and over a range of signal-to-noise levels ...
Cao, Ruijie, Li, Yaning, Chen, Xin, Ge, Xichuan, Li, Meiqi, Guan, Meiling, Hou, Yiwei, Fu, Yunzhe, Xu, Xinzhu, Leterrier, Christophe, Jiang, Shan, Gao, Baoxiang, Xi, Peng   +12 more
openaire   +4 more sources

OpenSpinMicroscopy: an open-source integrated microscopy platform

Nature Methods, 2013
Light-sheet microscopy has recently emerged as the technique of choice for fast, high-resolution three-dimensional (3D) imaging of whole organisms, one that also features low photodamage1,2. Unlike confocal or multiphoton systems, light sheet–based microscopes can easily provide a combination of multiple views by rotating the sample.
Emilio J, Gualda, Tiago, Vale, Pedro, Almada, José A, Feijó, Gabriel G, Martins, Nuno, Moreno   +5 more
openaire   +6 more sources

DeconvolutionLab2: An open-source software for deconvolution microscopy

Methods, 2017
Images in fluorescence microscopy are inherently blurred due to the limit of diffraction of light. The purpose of deconvolution microscopy is to compensate numerically for this degradation. Deconvolution is widely used to restore fine details of 3D biological samples. Unfortunately, dealing with deconvolution tools is not straightforward. Among others,
Sage, Daniel, Donati, Lauréne, Soulez, Ferréol, Fortun, Denis, Schmit, Guillaume, Seitz, Arne, Guiet, Romain, Vonesch, Cédric, Unser, Michael   +8 more
openaire   +5 more sources

Microscopy Nodes: versatile 3D microscopy visualization with Blender [PDF]

EMBO Reports
Effective visualization of 3D microscopy data is essential for communicating biological results. While scientific 3D rendering software is specifically designed for this purpose, it often lacks the flexibility found in non-scientific software like ...
Aafke Gros, Chandni Bhickta, Granita Lokaj, Brady Johnston, Yannick Schwab, Simone Köhler, Niccolò Banterle   +6 more
doaj   +2 more sources

One-step preparation of antimicrobial silicone materials based on PDMS and salicylic acid: insights from spatially and temporally resolved techniques

npj Biofilms and Microbiomes, 2021
In this work, we introduce a one-step strategy that is suitable for continuous flow manufacturing of antimicrobial PDMS materials. The process is based on the intrinsic capacity of PDMS to react to certain organic solvents, which enables the ...
Luca Barbieri, Ioritz Sorzabal Bellido, Alison J. Beckett, Ian A. Prior, Jo Fothergill, Yuri A. Diaz Fernandez, Rasmita Raval   +6 more
doaj   +1 more source

Effect of Local Topography on Cell Division of Staphylococcus spp.

Nanomaterials, 2022
Surface engineering is a promising strategy to limit or prevent the formation of biofilms. The use of topographic cues to influence early stages of biofilm formationn has been explored, yet many fundamental questions remain unanswered.
Ioritz Sorzabal-Bellido, Luca Barbieri, Alison J. Beckett, Ian A. Prior, Arturo Susarrey-Arce, Roald M. Tiggelaar, Joanne Fothergill, Rasmita Raval, Yuri A. Diaz Fernandez   +8 more
doaj   +1 more source

mmSIM: An open toolbox for accessible structured illumination microscopy [PDF]

Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, 2021
SummarySince the first practical super-resolution structured illumination fluorescence microscopes (SIM) were demonstrated more than two decades ago the method has become increasingly popular for a wide range of bioimaging applications. The high cost and relative inflexibility of commercial systems, coupled with the conceptual simplicity of the ...
Craig T. Russell, Michael Shaw
openaire   +4 more sources

Open microscopy in the life sciences: quo vadis?

Nature Methods, 2022
Light microscopy allows observing cellular features and objects with sub-micrometer resolution. As such, light microscopy has been playing a fundamental role in the life sciences for more than a hundred years. Fueled by the availability of mass-produced electronics and hardware, publicly shared documentation and building instructions, open-source ...
Johannes Hohlbein, Benedict Diederich, Barbora Marsikova, Emmanuel G. Reynaud, Séamus Holden, Wiebke Jahr, Robert Haase, Kirti Prakash   +7 more
openaire   +4 more sources

The K2: Open-source simultaneous triple-color TIRF microscope for live-cell and single-molecule imaging

HardwareX, 2023
Imaging the dynamics and interactions of biomolecules at the single-molecule level in live cells and reconstituted systems has generated unprecedented knowledge about the biomolecular processes underlying many cellular functions. To achieve the speed and
Christian Niederauer, Marco Seynen, Jan Zomerdijk, Marko Kamp, Kristina A. Ganzinger   +4 more
doaj   +1 more source