Results 81 to 90 of about 1,366,220 (285)

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Microscope Upcycling: Transforming legacy microscopes into automated cloud-integrated imaging systems

HardwareX
Computerized microscopes improve repeatability, throughput, antisepsis, data analysis and data sharing in the biological laboratory, but these machines are cost-prohibitive in most academic environments.
Drew Ehrlich, Yohei Rosen, David F. Parks, Kivilcim Doganyigit, Ryan Fenimore, Samira Vera-Choqqueccota, Sebastian Hernandez, Anna Toledo, David Haussler, Sri Kurniawan, Mircea Teodorescu   +10 more
doaj   +1 more source

Conducting interfaces between band insulating oxides: the LaGaO3/SrTiO3

, 2010
We show that the growth of the heterostructure LaGaO3/SrTiO3 yields the formation of a highly conductive interface. Our samples were carefully analyzed by high resolution electron microscopy, in order to assess their crystal perfection and to evaluate ...
Cantoni, Claudia, Codda, Marta, di Uccio, Umberto Scotti, Gazquez, Jaume, Granozio, Fabio Miletto, Maccariello, Davide, Marré, Daniele, Pallecchi, Ilaria, Pennycook, Steve, Perna, Paolo, Radovic, Milan, Varela, Maria   +11 more
core   +1 more source

Open Microscopy in Challenge-Based Learning

The Biophysicist
ABSTRACT Microscopy is an essential technique in biology, chemistry, and physics. Therefore, training in microscopy is crucial in science education. However, there is a gap between theory and practice because microscopes are perceived as “black boxes” rather than instruments that use geometrical and wave optics to provide an image ...
Marrit M. E. Tholen, Tom C. van Hattem, Lorenzo Albertazzi   +2 more
openaire   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Photonic bandgap plasmonic waveguides

, 2011
A novel type of a plasmonic waveguide has been proposed featuring an "open" design that is easy to manufacture, simple to excite and that offers a convenient access to a plasmonic mode.
Andrey B. Evlyukhin, Andrey Markov, Bora Ung, Boris N. Chichkov, Carsten Reinhardt, Grigorenko, Harris, Kabashin, Liedberg, Maksim Skorobogatiy, Radko, Reinhardt, Reinhardt, Sharma, Skorobogatiy, Wei Cheng, Zhang   +16 more
core   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Open-source sub-nanometer stabilization system for super-resolution fluorescence microscopy

Light: Science & Applications
Recent advances in fluorescence nanoscopy have pushed resolution to the 1–10 nm range, enabling the direct visualization of individual molecules even in crowded biological environments.
Florencia Edorna, Florencia D. Choque, Giovanni Ferrari, Luciano A. Masullo, Piotr Zdańkowski, Guillermo P. Acuna, Philip Tinnefeld, Alan M. Szalai, Lucía F. Lopez, Andrés Zelcer, Fernando D. Stefani   +10 more
doaj   +1 more source

Strain superlattices and macroscale suspension of Graphene induced by corrugated substrates

, 2014
We investigate the organized formation of strain, ripples and suspended features in macroscopic CVD-prepared graphene sheets transferred onto a corrugated substrate made of an ordered arrays of silica pillars of variable geometries.
Autier-Laurent, Sandrine, Autier-Laurent, Sandrine, Bendiab, Nedjma, Bouchiat, Hélène, Bouchiat, Vincent, Ferlazzo, Laurence, Guéron, Sophie, Han, Zheng, Kalita, Dipankar, Komatsu, Katsuyoshi, Li, Chuan, Marty, Laetitia, Ralko, Arnaud, Reserbat-Plantey, Antoine, Weil, Raphaël   +14 more
core   +3 more sources

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

FEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga, Toshiki Takahashi, Akiko Kuma, Hiroyuki Kawahara   +3 more
wiley   +1 more source