Results 161 to 170 of about 1,658,706 (311)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Does respiratory co-infection facilitate dispersal of SARS-CoV-2? Investigation of a super-spreading event in an open-space office [PDF]

Antimicrob Resist Infect Control, 2020
Weissberg D, Böni J, Rampini S, Kufner V, Zaheri M, Schreiber PW, Abela IA, Huber M, Sax H, Wolfensberger A.   +9 more
europepmc   +2 more sources

What are the topics you care about making trials in lupus more effective? Results of an Open Space meeting of international lupus experts. [PDF]

Lupus Sci Med, 2021
Mucke J, Alarcon-Riquelme M, Andersen J, Aringer M, Bombardieri S, Brinks R, Cervera R, Chehab G, Cornet A, Costedoat-Chalumeau N, Czirják L, Doria A, Fischer-Betz R, Furie RA, Gatto M, Houssiau FA, Ines L, Liang MH, Morand E, Mosca M, Pego-Reigosa JM, Rúa-Figueroa I, Ruiz-Irastorza G, Terrier B, Voss A, Schneider M.   +25 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Examining privilege and power in US urban parks and open space during the double crises of antiblack racism and COVID-19. [PDF]

Socioecol Pract Res, 2021
Hoover FA, Lim TC.
europepmc   +1 more source

»Open Space«

, 2011
Künstlerische Forschung bedeutet am Institut für Musik und Akustik (IMA) des ZKM zugleich meist technologische Forschung und Entwicklung. Die Produktionen sind einerseits durch eine heterogene und individuelle Arbeitweise der Gastkomponisten geprägt, andererseits kanalisiert das IMA durch übergeordnete künstlerische und wissenschaftliche Fragestellung (
openaire   +2 more sources

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source