Results 201 to 210 of about 1,541,414 (259)
FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
FEBS Open Bio, EarlyView.Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
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Information, Communication & Society, 2009
This article addresses open source software development and the open source movement and critically examines their utility as a metaphor for conceptualizing the new politics of networked organizations. We apply the open source metaphor to the World Social Forum (WSF), which in terms of its Charter of Principles, and its notion of open, horizontal and ...
Peter J. Smith, Elizabeth Smythe
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This article addresses open source software development and the open source movement and critically examines their utility as a metaphor for conceptualizing the new politics of networked organizations. We apply the open source metaphor to the World Social Forum (WSF), which in terms of its Charter of Principles, and its notion of open, horizontal and ...
Peter J. Smith, Elizabeth Smythe
openaire +1 more source
Communications of the ACM, 2010
The U.S. Federal Communications Commission's decision to open frequencies in the broadcast spectrum could enable broadband networks in rural areas, permit smart electric grids, and more.
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The U.S. Federal Communications Commission's decision to open frequencies in the broadcast spectrum could enable broadband networks in rural areas, permit smart electric grids, and more.
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Classical Antiquity, 2020
This paper explores the construction of dramatic space in the prologues of classical Greek drama. Drawing from theater scholarship on the phenomenology of space, I show how tragedians and comedians alike experimented with how to shape their audience’s understanding of a play’s setting.
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This paper explores the construction of dramatic space in the prologues of classical Greek drama. Drawing from theater scholarship on the phenomenology of space, I show how tragedians and comedians alike experimented with how to shape their audience’s understanding of a play’s setting.
openaire +1 more source
From Fragmented Open Space to Networked Open Space
2014Session B – Panel III : Subtheme 4: Social inclusion/exclusion, spatial segregation and fragmented ...
Cheung, MW, Tang, BS
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City, 2018
This paper contributes to debates on urban citizenship and public culture. Drawing on detailed empirical research in Istanbul, Turkey, we analyse two key contemporary arts organisations that experiment with new organisational and curatorial practices in order to realise cultural infrastructures of common life in a city strongly shaped by urban ...
Sanul, Rukiye Gökçe, van Heur, Bas
openaire +2 more sources
This paper contributes to debates on urban citizenship and public culture. Drawing on detailed empirical research in Istanbul, Turkey, we analyse two key contemporary arts organisations that experiment with new organisational and curatorial practices in order to realise cultural infrastructures of common life in a city strongly shaped by urban ...
Sanul, Rukiye Gökçe, van Heur, Bas
openaire +2 more sources
2017
An examination of environmental satellite data sharing policies, offering a model of data-sharing policy development, case and practical recommendations for increasing global data sharing. Key to understanding and addressing climate change is continuous and precise monitoring of environmental conditions. Satellites play an important role
openaire +1 more source
An examination of environmental satellite data sharing policies, offering a model of data-sharing policy development, case and practical recommendations for increasing global data sharing. Key to understanding and addressing climate change is continuous and precise monitoring of environmental conditions. Satellites play an important role
openaire +1 more source