Results 101 to 110 of about 521,948 (283)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Operator-space Grothendieck inequalities for noncommutative Lp-spaces [PDF]

open access: yesDuke Mathematical Journal, 2006
We prove the operator space Grothendieck inequality for bilinear forms on subspaces of noncommutative $L_p$-spaces with ...
openaire   +4 more sources

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

open access: yesFEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon   +6 more
wiley   +1 more source

Enzymatic degradation of biopolymers in amorphous and molten states: mechanisms and applications

open access: yesFEBS Open Bio, EarlyView.
This review explains how polymer morphology and thermal state shape enzymatic degradation pathways, comparing amorphous and molten biopolymer structures. By integrating structure–reactivity principles with insights from thermodynamics and enzyme engineering, it highlights mechanisms that enable efficient polymer breakdown.
Anđela Pustak, Aleksandra Maršavelski
wiley   +1 more source

Precompact Sets, Boundedness, and Compactness of Commutators for Singular Integrals in Variable Morrey Spaces

open access: yesJournal of Function Spaces, 2017
We give sufficient conditions for subsets to be precompact sets in variable Morrey spaces. Then we obtain the boundedness of the commutator generated by a singular integral operator and a BMO function on the variable Morrey spaces.
Wei Wang, Jingshi Xu
doaj   +1 more source

Characterizations of Ordered Self-adjoint Operator Spaces

open access: yes, 2016
In this paper, we generalize the work of Werner and others to develop two abstract characterizations for self-adjoint operator spaces. The corresponding abstract objects can be represented as self-adjoint subspaces of $B(H)$ in such a way that both a ...
Russell, Travis
core  

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Local spectral radius formulas for a class of unbounded operators on Banach spaces

open access: yes, 2011
We exhibit a general class of unbounded operators in Banach spaces which can be shown to have the single-valued extension property, and for which the local spectrum at suitable points can be determined. We show that a local spectral radius formula holds,
Andersen, Nils Byrial, de Jeu, Marcel
core  

The role of lipid metabolism in neuronal senescence

open access: yesFEBS Open Bio, EarlyView.
Disrupted lipid metabolism, through alterations in lipid species or lipid droplet accumulation, can drive neuronal senescence. However, lipid dyshomeostasis can also occur alongside neuronal senescence, further amplifying tissue damage. Delineating how lipid‐induced senescence emerges in neurons and glial cells, and how it contributes to ageing and ...
Dikaia Tsagkari   +2 more
wiley   +1 more source

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