Results 51 to 60 of about 114,748 (203)

Gene expression noise in a complex artificial toxin expression system.

PLoS ONE, 2020
Gene expression is an intrinsically stochastic process. Fluctuations in transcription and translation lead to cell-to-cell variations in mRNA and protein levels affecting cellular function and cell fate. Here, using fluorescence time-lapse microscopy, we
Alexandra Goetz, Andreas Mader, Benedikt von Bronk, Anna S Weiss, Madeleine Opitz   +4 more
doaj   +1 more source

Streptavidins Coordinate Biotin Sequestration and Self‐Resistance Within a Biotin‐Pathway Antibiotic Network

Advanced Science, EarlyView.
A conserved genomic region between two streptavidin genes in Streptomyces packages biosynthetic gene clusters for diverse biotin‐pathway antibiotics: acidomycin, stravidin, the new non‐proteinogenic amino acid ANDA, and the new BioA inhibitor α‐methyl‐KAPA.
Sumire Kurosawa, Jingjun Mo, Xiaotong Diao, Feng Xie, Tingting Wang, Haowen Zhao, Dalei Wu, Rolf Müller, Chengzhang Fu   +8 more
wiley   +1 more source

The life-cycle of operons. [PDF]

PLoS Genetics, 2006
Operons are a major feature of all prokaryotic genomes, but how and why operon structures vary is not well understood. To elucidate the life-cycle of operons, we compared gene order between Escherichia coli K12 and its relatives and identified the ...
Morgan N Price, Adam P Arkin, Eric J Alm
doaj   +1 more source

Identification and Molecular Characterization of the Operon Required for L-Asparagine Utilization in Corynebacterium glutamicum

Microorganisms, 2022
Understanding the metabolic pathways of amino acids and their regulation is important for the rational metabolic engineering of amino acid production.
Koichi Toyoda, Riki Sugaya, Akihiro Domon, Masako Suda, Kazumi Hiraga, Masayuki Inui   +5 more
doaj   +1 more source

ProSiteHunter: A Unified Framework for Sequence‐Based Prediction of Protein‐Nucleic Acid and Protein‐Protein Binding Sites

Advanced Science, EarlyView.
This study proposed a unified sequence‐based framework for protein binding site prediction, which adopted a tri‐track semantic multi‐source feature fusion strategy to effectively capture diverse macromolecular interaction sites and further improved the accuracy of antibody‐antigen interaction prediction.
Dongliang Hou, Qihang Zhen, Zexin Lv, Xinyue Cui, Suhui Wang, Minghua Hou, Zhan Zhou, Xiaogen Zhou, Guijun Zhang   +8 more
wiley   +1 more source

Identification of anthranilate and benzoate metabolic operons of Pseudomonas fluorescens and functional characterization of their promoter regions

Microbial Cell Factories, 2006
Background In an effort to identify alternate recombinant gene expression systems in Pseudomonas fluorescens, we identified genes encoding two native metabolic pathways that were inducible with inexpensive compounds: the anthranilate operon (antABC) and ...
Lee Vincent D, Resnick Sol M, Haney Keith L, Shao Ying, Thomas Tracey C, Retallack Diane M, Squires Charles H   +6 more
doaj   +1 more source

CREAT: A CRISPR‐Based Genome Trimming Strategy for Systematic Identification of Dispensable Regions and Rapid Genome Reduction

Advanced Science, EarlyView.
Current genome‐reduction methods work in a trial‐and‐error fashion, constituting a long‐standing bottleneck in the research. We developed CREAT (CRISPR‐based genome trimming with a multi‐homology‐arm template), a CRISPR‐based genetic approach that enables systematically classifying essential versus non‐essential genomic regions and subsequently ...
Guanhua Yuan, Zhe Gao, Yingxuan Qi, Yang Zhang, Xuhui Tian, Pengpeng Zhao, Xu Feng, Qunxin She   +7 more
wiley   +1 more source

High Tree Species Diversity Promotes Thermal Enhancement Response of Microbial Carbon Use Efficiency

Advanced Science, EarlyView.
ABSTRACT Predicting soil carbon dynamics under warming is constrained by limited understanding of microbial thermal adaptation, particularly whether microbial carbon use efficiency (CUE) can adapt to warming and how plant diversity modulates this response. Using soils from a natural tree species diversity gradient in a subtropical forest, we combined a
Pengpeng Duan, Changqing Ye, Xinyi Yang, Chaoqun Wang, Wolfgang Wanek, Jian‐sheng Ye, Hongzhao Yuan, Hu Du, Kongcao Xiao, Xunyang He, Kelin Wang, Dejun Li   +11 more
wiley   +1 more source

Using origami and Shrinky Dinks to create active learning activities to tackle two microbiology concepts: cell structure differences and operon regulation

Journal of Microbiology & Biology Education
This paper presents two low-cost hands-on activities designed to enhance student understanding and address the pedagogical challenges faced by microbiology professors in teaching concepts related to cell structure and gene regulation.
Manuela Tripepi, Hannah M. Schapiro
doaj   +1 more source

Pyrazole‐Derived Antibacterial Compounds Effectively Treat Methicillin‐Resistant Staphylococcus Aureus Infections by Inhibiting Aspartate Transcarbamoylase

Advanced Science, EarlyView.
This study unveils a novel antibacterial mechanism against MRSA, in which the compound Py‐27 selectively targets and inhibits aspartate transcarbamoylase (ATCase), a key enzyme in pyrimidine synthesis. This inhibition disrupts DNA replication, induces oxidative damage, leading to potent bactericidal activity.
Xiaorong Yang, Xinyuan Cao, Lixia Dai, Feng Yang, Jing Wang, Yudong Ma, Tong Bu, Zile Gong, Xueyan Liu, Changcai Bai, Xiaolou Miao, Xiaofei Shang   +11 more
wiley   +1 more source