Results 61 to 70 of about 146,124 (285)

Tracing the ancestry of operons in bacteria [PDF]

Bioinformatics, 2017
Abstract Motivation Complexity is a fundamental attribute of life. Complex systems are made of parts that together perform functions that a single component, or subsets of components, cannot. Examples of complex molecular systems include protein structures such as the F1Fo-ATPase, the ribosome,
Huy N. Nguyen, Ashish Jain, Oliver Eulenstein, Iddo Friedberg   +3 more
openaire   +2 more sources

Tools for characterization of Escherichia coli genes of unknown function [PDF]

, 2002
Despite the power of sequencing and of emerging high-throughput technologies to collect data rapidly, the definitive functional characterization of unknown genes still requires biochemical and genetic analysis in case-by-case studies. This often involves
Masters, Millicent, Merlin, Christophe, McAteer, Sean, Masters, Millicent/Mrs Donachie   +3 more
core   +1 more source

De Novo Autogenic Engineered Living Functional Materials

Advanced Materials, EarlyView.
A versatile platform for de novo autogenic engineered living materials is presented, leveraging protein mining, computational modeling, and synthetic biology. By reprogramming the Escherichia coli curli machinery with β‐solenoid proteins from non‐model bacteria, macroscopic biomaterials with enhanced mechanical properties, UV‐C irradiation protection ...
Hoda M. Hammad, Seth Swarnadeep, Erin C. Jackson, Nicolas Burns, Hongyu Wang, Harrison Priode, Robert B. Moore, Sanket Deshmukh, Avinash Manjula‐Basavanna, Anna M. Duraj‐Thatte   +9 more
wiley   +1 more source

Beyond Canonical CO Oxidation: Structural and Evolutionary Insights Into a Non‐Canonical Carbon Monoxide Dehydrogenase

Angewandte Chemie, EarlyView.
Clade B carbon monoxide dehydrogenases (CODHs) remain enigmatic, diverging from canonical CODHs in structure and function. Here, we present the first characterization of Ruminococcus flavefaciens CODH (RfCODH), revealing occluded substrate pathways and attenuated CO oxidation activity.
Maximilian Böhm, Vivek Srinivas, Benjamin Wiseman, Ping Huang, Moritz Senger, Martin Högbom, Henrik Land   +6 more
wiley   +2 more sources

An inducible fusaric acid tripartite efflux pump contributes to the fusaric acid resistance in Stenotrophomonas maltophilia. [PDF]

PLoS ONE, 2012
BACKGROUND: Fusaric acid (5-butylpicolinic acid), a mycotoxin, is noxious to some microorganisms. Stenotrophomonas maltophilia displays an intrinsic resistance to fusaric acid.
Rouh-Mei Hu, Sih-Ting Liao, Chiang-Ching Huang, Yi-Wei Huang, Tsuey-Ching Yang   +4 more
doaj   +1 more source

Identification and Molecular Characterization of the Operon Required for L-Asparagine Utilization in Corynebacterium glutamicum

Microorganisms, 2022
Understanding the metabolic pathways of amino acids and their regulation is important for the rational metabolic engineering of amino acid production.
Koichi Toyoda, Riki Sugaya, Akihiro Domon, Masako Suda, Kazumi Hiraga, Masayuki Inui   +5 more
doaj   +1 more source

Transcription Factor Promiscuity Drives Regulatory Rewiring and Evolvability in Gene Networks in Bacteria

Advanced Science, EarlyView.
When a master transcription factor (TF) is lost, bacteria can rapidly rewire gene regulatory networks by co‐opting related regulators. Using experimental evolution in Pseudomonas fluorescens, we show that TF promiscuity (low‐level, non‐cognate binding) provides the raw material for rewiring. Successful co‐option follows a predictable hierarchy governed
Tiffany B. Taylor, Alan M. Rice
wiley   +1 more source

A Conserved Isoleucine Gates the Diffusion of CO and O2 to the Active Site of NiFe CO‐Dehydrogenase

Angewandte Chemie, EarlyView.
A combination of protein film electrochemistry and site‐directed mutagenesis shows that isoleucine 563 (numbering of Thermococcus sp. AM4) gates the arrival of both CO and O2 to the active site of the nickel‐iron CO dehydrogenases. Mutations at this position increase the resistance to O2 at the cost of a slower reaction with CO.
Laura Opdam, Marta Meneghello, Chloé Guendon, Jade Chargelègue, Andrea Fasano, Aurore Jacq‐Bailly, Christophe Léger, Vincent Fourmond   +7 more
wiley   +2 more sources

High Tree Species Diversity Promotes Thermal Enhancement Response of Microbial Carbon Use Efficiency

Advanced Science, EarlyView.
ABSTRACT Predicting soil carbon dynamics under warming is constrained by limited understanding of microbial thermal adaptation, particularly whether microbial carbon use efficiency (CUE) can adapt to warming and how plant diversity modulates this response. Using soils from a natural tree species diversity gradient in a subtropical forest, we combined a
Pengpeng Duan, Changqing Ye, Xinyi Yang, Chaoqun Wang, Wolfgang Wanek, Jian‐sheng Ye, Hongzhao Yuan, Hu Du, Kongcao Xiao, Xunyang He, Kelin Wang, Dejun Li   +11 more
wiley   +1 more source

Atypical Condensation Domains Guide Discovery and Illuminate Biosynthesis of Myxoglucamides Featuring Vinyl‐Substituted, α‐Oxidized γ‐Amino Acids

Angewandte Chemie, EarlyView.
Genome mining of atypical condensation (C) domains leads to the discovery of myxoglucamides, glycolipopeptides featuring an unprecedented vinyl‐substituted, α‐oxidized γ‐amino acid. Biosynthetic studies reveal a discrete C‐domain‐like O‐acyltransferase, a β‐hydroxylation‐dependent chain‐extension checkpoint, and an unusual pathway for γ‐amino acid ...
Tingting Wang, Alexander Popoff, Maja Hunter, Guangzhi Dai, Ronald Garcia, Huixin Xu, Haowen Zhao, Asfandyar Sikandar, Liujie Huo, Rolf Müller, Chengzhang Fu   +10 more
wiley   +2 more sources