Comparative transcriptomics and scarless genome editing uncover a periplasmic <i>c</i>-type cytochrome important for extracellular electron uptake in <i>Thioclava electrotropha</i> ElOx9<sup>T</sup>. [PDF]
Sackett JD +4 more
europepmc +1 more source
Nucleotide‐detected solid‐state NMR has been applied to probe nucleotide conformations and dynamics during ATP hydrolysis using the dimeric P‐loop ATPase SmsC as a model protein. The different stages of ATP hydrolysis have been mimicked by ATP analogues for which different degrees of conformational heterogeneity have been observed. ABSTRACT Solid‐state
Nina Wehr +7 more
wiley +1 more source
Regulation of multiple paralogs of a small subunit ribosomal protein in <i>Francisella tularensis</i>. [PDF]
Schmidt SS +5 more
europepmc +1 more source
A novel photodynamic therapeutic approach has been employed for anticancer therapy, utilizing chlorin e6‐loaded Pluronic nanocapsules and a wearable organic light‐emitting diode light source. This has shown significant in vivo antitumor efficacy with minimal off‐target toxicity. The innovative combination of a wearable light source and photosensitizers
Hyeryeon Oh +7 more
wiley +1 more source
Functional characterization of the oligopeptide transporter operon oppABCDF-1 in Escherichia coli Nissle 1917. [PDF]
Yu P, Sun R, Huang Y, Yang Y.
europepmc +1 more source
De Novo Autogenic Engineered Living Functional Materials
A versatile platform for de novo autogenic engineered living materials is presented, leveraging protein mining, computational modeling, and synthetic biology. By reprogramming the Escherichia coli curli machinery with β‐solenoid proteins from non‐model bacteria, macroscopic biomaterials with enhanced mechanical properties, UV‐C irradiation protection ...
Hoda M. Hammad +9 more
wiley +1 more source
Co-Directional Chromosomal Clustering of Metabolic Pathway Genes as a Layout Prior for Synthetic Construct Design. [PDF]
Han X, Qiu Z, Hu J, Song J.
europepmc +1 more source
Current genome‐reduction methods work in a trial‐and‐error fashion, constituting a long‐standing bottleneck in the research. We developed CREAT (CRISPR‐based genome trimming with a multi‐homology‐arm template), a CRISPR‐based genetic approach that enables systematically classifying essential versus non‐essential genomic regions and subsequently ...
Guanhua Yuan +7 more
wiley +1 more source
Lactic Acid Bacteria-Derived γ-Aminobutyric Acid: From Targeted Screening and Biosynthesis to Functional Food Applications and Health Benefits. [PDF]
Zhang Y +6 more
europepmc +1 more source
High Tree Species Diversity Promotes Thermal Enhancement Response of Microbial Carbon Use Efficiency
ABSTRACT Predicting soil carbon dynamics under warming is constrained by limited understanding of microbial thermal adaptation, particularly whether microbial carbon use efficiency (CUE) can adapt to warming and how plant diversity modulates this response. Using soils from a natural tree species diversity gradient in a subtropical forest, we combined a
Pengpeng Duan +11 more
wiley +1 more source

