Results 51 to 60 of about 146,400 (289)

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

The Rhizobium etli trpB Gene Is Essential for an Effective Symbiotic Interaction with Phaseolus vulgaris

Molecular Plant-Microbe Interactions, 1999
A mutant strain (CTNUX4) of Rhizobium etli carrying Tn5 unable to grow with ammonium as the sole nitrogen source was isolated and characterized. Sequence analysis showed that Tn5 is inserted into a trpB (tryptophan synthase)-homologous gene.
Rosarita Taté, Anna Riccio, Emilia Caputo, Michele Cermola, Renee Favre, Eduardo J. Patriarca   +5 more
doaj   +1 more source

The non-classical ArsR-family repressor PyeR (PA4354) modulates biofilm formation in Pseudomonas aeruginosa [PDF]

, 2012
PyeR (PA4354) is a novel member of the ArsR family of transcriptional regulators and modulates biofilm formation in Pseudomonas aeruginosa. Characterization of this regulator showed that it has negative autoregulatory properties and binds to a ...
Plower, E., Mooij, M., McCarthy, R., Wang, Y. P., Dobson, A., O'Gara, F., O'Gara, F., Mac Aogáin, M., Tian, Z.X., Morrissey, J., Wang, Y.P., Tian, Z., Adams, C., McCarthy, R.R., McCarthy, R. R., Wang, Y., Tian, Z. X., O'Gara, Fergal, Mooij, M.J., Mooij, M. J.   +19 more
core   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Development of an sRNA-mediated conditional knockdown system for Chlamydia trachomatis

mBio
We describe a new Chlamydia trachomatis protein depletion method that uses an engineered small RNA (sRNA) to inhibit translation of a target gene. In proof-of-principle experiments, we induced functional knockdown of IncA, a fusion-mediating inclusion ...
Janina Ehses, Kevin Wang, Asha Densi, Cuper Ramirez, Ming Tan, Christine Sütterlin   +5 more
doaj   +1 more source

Horizontal gene transfer and diverse functional constrains within a common replication-partitioning system in Alphaproteobacteria: the repABC operon

BMC Genomics, 2009
Background The repABC plasmid family, which is extensively present within Alphaproteobacteria, and some secondary chromosomes of the Rhizobiales have the particular feature that all the elements involved in replication and partitioning reside within one ...
Cevallos Miguel A, González Víctor, Vázquez-Castellanos Jorge F, Castillo-Ramírez Santiago   +3 more
doaj   +1 more source

Operon and non-operon gene clusters in the C. elegans genome [PDF]

WormBook, 2014
Nearly 15% of the ~20,000 C. elegans genes are contained in operons, multigene clusters controlled by a single promoter. The vast majority of these are of a type where the genes in the cluster are ~100 bp apart and the pre-mRNA is processed by 3' end formation accompanied by trans-splicing.
Thomas, Blumenthal, Paul, Davis, Alfonso, Garrido-Lecca   +2 more
openaire   +2 more sources

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Salmonella enterica serovar typhi limits the potency of typhoid toxin and ADP‐ribosylating toxin AB to establish a persistent infection

FEBS Open Bio, EarlyView.
The two catalytic subunits of typhoid toxin dissociate from the holotoxin in the ER of an intoxicated cell, but only CdtB exits the ER to generate immunosuppressive effects. PltA is retained in the ER and sequestered from its cytosolic target, thus allowing the anti‐inflammatory effects of CdtB to promote intestinal colonization.
Maria C. Zabala‐Rodriguez, Juliette K. Tinker, Teresa Frisan, Taj Azarian, Ken Teter   +4 more
wiley   +1 more source

Investigation of carbon catabolite repression in Clostridium beijerinckii NCIMB 8052 [PDF]

, 2001
Summary in English.Includes bibliographical references.The substrate basis for the industrial acetone-butanol-ethanol (ABE) fermentations, has been agricultural products rich in starch or sucrose, and employed taxonomically distinct amylolytic and ...
Rafudeen, M S
core   +1 more source