Results 201 to 210 of about 118,072 (257)

RNA‐Guided Engineering of the Chloroplast Genome Enabled by Plastid‐Expressed Guide RNAs

open access: yesPlant Biotechnology Journal, Volume 24, Issue 10, Page 5498-5509, October 2026.
ABSTRACT Our goal is to develop RNA‐guided engineering of the chloroplast genome using the CRISPR/Cas9 system. We designed chloroplast minigenes to obtain properly sized single guide RNAs (sgRNAs) in tobacco chloroplasts. The sgRNA 5′ end is defined by transcription from an rRNA operon promoter, and its 3′ end by processing a downstream tRNA (trnG) or ...
Malihe Mirzaee   +3 more
wiley   +1 more source

Exploring the metabolic burden of surfactin biosynthesis and the metabolic costs of srfA operon expression in Bacillus subtilis. [PDF]

open access: yesMicrob Cell Fact
Perino EHB   +8 more
europepmc   +1 more source

Evolution‐Inspired Engineering of Diterpene Biosynthesis via Chloroplast Genome Modification

open access: yesPlant Biotechnology Journal, Volume 24, Issue 10, Page 5510-5525, October 2026.
ABSTRACT Terpenes constitute the largest and most structurally diverse class of plant secondary metabolites, with critical roles in plant‐environment interactions and broad industrial applications. Although nuclear genome engineering of terpene pathways has been extensively explored, chloroplast genome engineering remains largely undeveloped, with most
Alessandro Occhialini   +6 more
wiley   +1 more source

A bacterial pangenome association study identifies a role for polyamine metabolism and alkaline tolerance in middle ear infections. [PDF]

open access: yesPLoS Pathog
Hammond JA   +19 more
europepmc   +1 more source

Characterizing the pangenome-based virulome and resistome of uropathogenic <i>Escherichia coli</i> from Ecuador. [PDF]

open access: yesVirulence
Mejía-Limones I   +3 more
europepmc   +1 more source

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