Results 91 to 100 of about 114,748 (203)
Metagenomic Evaluation of Oral Microbiota in Patients Affected by Oral Lichen Planus: A Pilot Study
ABSTRACT Objective To characterize the oral bacterial and fungal microbiota of symptomatic oral lichen planus patients undergoing topical corticosteroid therapy and to explore microbial patterns potentially associated with subsequent oral candidiasis. Methods Twelve patients with clinically and histologically confirmed OLP were enrolled.
A. Dormiente +5 more
wiley +1 more source
The ComEA/ComEC Operon is Critical for Nitrosative Stress Tolerance by an Oral Commensal
ABSTRACT Oral commensal streptococci play a large role in mediating microbial homeostasis. Our prior work has demonstrated that hydrogen peroxide (H2O2) produced by oral commensal streptococci can react with salivary nitrite (NO2) and generate peroxynitrite (ONOO−), a potent antimicrobial.
Sara E. Edmonds +3 more
wiley +1 more source
ABSTRACT The single crossover occurring via homologous recombination is a common phenomenon existing among microbes like Escherichia coli, Bacillus subtilis, Vibrio natriegens, Gluconobacter oxydans and most cyanobacteria species, threatening the stability of engineered strains and challenging iterative genetic engineering. Among them, we take the fast‐
Shubin Li +5 more
wiley +1 more source
RNA‐Guided Engineering of the Chloroplast Genome Enabled by Plastid‐Expressed Guide RNAs
ABSTRACT Our goal is to develop RNA‐guided engineering of the chloroplast genome using the CRISPR/Cas9 system. We designed chloroplast minigenes to obtain properly sized single guide RNAs (sgRNAs) in tobacco chloroplasts. The sgRNA 5′ end is defined by transcription from an rRNA operon promoter, and its 3′ end by processing a downstream tRNA (trnG) or ...
Malihe Mirzaee +3 more
wiley +1 more source
Evolution‐Inspired Engineering of Diterpene Biosynthesis via Chloroplast Genome Modification
ABSTRACT Terpenes constitute the largest and most structurally diverse class of plant secondary metabolites, with critical roles in plant‐environment interactions and broad industrial applications. Although nuclear genome engineering of terpene pathways has been extensively explored, chloroplast genome engineering remains largely undeveloped, with all ...
Alessandro Occhialini +6 more
wiley +1 more source
ABSTRACT Photosynthetic hydrogen (photoH2) production is a promising approach for the utilisation of solar energy. Fusion of a hydrogenase (H2ase) to photosystem I (PSI) provides a direct light‐driven route for photoH2 generation. We established the lateral transmembrane subunit PsaF of PSI as a new fusion partner for the NiFe‐hydrogenase (HoxYH) in ...
Florian Paul +4 more
wiley +1 more source
Advances in large DNA fragment assembly for microbial cell factory engineering
Abstract The efficient, rapid, and reliable assembly of DNA fragments is essential for advancing metabolic engineering and synthetic biology. With the rapid advancement of DNA synthesis and assembly technologies, the scale of DNA assembly has expanded from single genes to metabolic pathways and even genomes.
Yu Zhang +5 more
wiley +1 more source
ABSTRACT Sugarcane is one of the most important crops, providing the majority of global sugar production and serving as a major feedstock for bioenergy. Increasing demands for sustainable sugar production under climate and productivity constraint have intensified the need for molecular‐level characterization of elite cultivars.
Widhi Dyah Sawitri +6 more
wiley +1 more source
Syndromic convergence (SyCo) reframes aquaculture disease as the synergistic product of climate‐driven stressors, pathobiome communities, and host immune compromise, not single pathogens. A five‐criterion scoring matrix, composite early warning index, and three‐layer intervention framework together operationalize this shift toward predictive ...
Ilhan Altinok
wiley +1 more source
This study evaluates the impact of aguamiel syrup on a synbiotic fermented dairy product, focusing on antihypertensive peptide release and Lacticaseibacillus rhamnosus GG survival. While metabolic parameters remained unchanged, aguamiel syrup enhanced bioactive peptide production and antihypertensive activity (45.3%), suggesting its potential as a ...
Olvera‐Rosales Laura Berenice +6 more
wiley +1 more source

