Deep Mutational Scanning for the Study and Engineering of Protein Assemblies
This review explores how deep mutational scanning (DMS) can be used to improve understanding and accelerate engineering of protein assemblies, from natural fibers to engineered nanocages. We discuss various types of protein assemblies and their characterization, provide an introduction to the DMS technique, and then highlight examples in which DMS has ...
Jenna B. Wolfanger +2 more
wiley +1 more source
Spatial‐Scale Evolutionary Bias Sheds Light on the Latitudinal Diversity Gradient
Using Escherichia coli as a model species, our experimental findings reveal that populations in high‐fitness‐gain geographic regions undergo rapid expansion, competitively excluding populations from low‐fitness‐gain regions. This results in adaptive evolution that is biased toward areas with higher fitness potential.
Haoyuan Wu, Donghui Xu, Yonghua Wu
wiley +1 more source
Transformer-based operon prediction using textual representations of gene pairs. [PDF]
Assaf R, Fakhri B.
europepmc +1 more source
This study evaluates the impact of aguamiel syrup on a synbiotic fermented dairy product, focusing on antihypertensive peptide release and Lacticaseibacillus rhamnosus GG survival. While metabolic parameters remained unchanged, aguamiel syrup enhanced bioactive peptide production and antihypertensive activity (45.3%), suggesting its potential as a ...
Olvera‐Rosales Laura Berenice +6 more
wiley +1 more source
Expression of the <i>ydaJKLMN</i> operon increases the adhesion of <i>Bacillus subtilis</i> spores to biotic and abiotic surfaces. [PDF]
De Stefano M +3 more
europepmc +1 more source
Miniaturized Droplet‐Based Adaptation of the Ames Test for High‐Throughput Mutagenicity Assessment
ABSTRACT This work presents the first demonstration of a tube‐based droplet microfluidic implementation of the Ames test, bridging single‐droplet resolution with regulatory genotoxicity testing. The Ames test is a cornerstone assay for detecting mutagenicity, but conventional plate‐ and well‐based formats suffer from high reagent consumption, low ...
Jialan Cao +3 more
wiley +1 more source
Functions of the prp operon in carbon source metabolism and acetic acid resistance in acetic acid bacteria: a case study in Acetobacter pasteurianus CGMCC 1.41. [PDF]
Yang H, Ehrenreich A, Liebl W, Chen F.
europepmc +1 more source
The Dual Role of the Gut Microbiota in Cancer Chemoresistance
Comparison between Microbiota and Cancer therapy. ABSTRACT Chemoresistance is one of the primary reasons that cancer chemotherapy fails to deliver successful treatment outcomes and contributes to poor overall survival rates for patients with cancer. New research has begun to shed light on the effects of the gut microbiome (GM).
Hamed Tahmasebi +3 more
wiley +1 more source
Exploring the metabolic burden of surfactin biosynthesis and the metabolic costs of srfA operon expression in Bacillus subtilis. [PDF]
Perino EHB +8 more
europepmc +1 more source
Dry‐season pollutant concentration drives microbial restructuring in Bangladesh's Shitalakshya River, shifting communities from Myroides to stress‐tolerant genera while enriching metal resistance genes and efflux pumps, revealing non‐antibiotic co‐selection as a key driver of antimicrobial resistance in urban waters.
Muhammad Ehteshamul Haque +3 more
wiley +1 more source

