Results 41 to 50 of about 3,866,750 (249)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Bio-Chemical Sensors Based on Excessively Tilted Fiber Grating

Photonic Sensors
Excessively tilted fiber gratings (ExTFGs) are a type of special optical fiber grating device different from traditional fiber Bragg gratings, long period fiber gratings, and tilted fiber Bragg gratings.
Fudan Chen, Hong Gu, Binbin Luo, Shenghui Shi   +3 more
doaj   +1 more source

Steel Wire Rope Damage Width Identification Method Based on Residual Networks and Multi-Channel Feature Fusion

Machines
In order to ensure the safety of steel wire rope in various application scenarios, it is particularly important to quantitatively detect the defects of wire rope. Complex detection conditions affect the detection efficiency of wire rope. Therefore, based
Yan Peng, Junde Liu, Junjie He, Yongjun Qiu, Xie Liu, Le Chen, Fengfeng Yang, Bulong Chen, Bin Tang, Yuhan Wang   +9 more
doaj   +1 more source

KDAC6 alters cell morphology and motility through positive and negative modulation of F‐actin distribution

FEBS Open Bio, EarlyView.
KDAC6 has been associated with cell motility and actin structures, but specific domain contributions are not established. The two catalytic domains have differential effects on motility and F‐actin regulation, affecting cortical F‐actin density, motility, stress fibers, and cell spreading.
Taylor V. Joseph, Andrea B. Pham, Kiara E. Bornes, Razan H. Hammad, Chelsea A. Rousseve‐Ross, Thomas M. Huckaba, Terry J. Watt, Tasha B. Toro   +7 more
wiley   +1 more source

Research on the surface subsidence monitoring technology based on fiber Bragg grating sensing

Photonic Sensors, 2016
In order to monitor the process of surface subsidence caused by mining in real time, we reported two types of fiber Bragg grating (FBG) based sensors.
Jinyu Wang, Long Jiang, Zengrong Sun, Binxin Hu, Faxiang Zhang, Guangdong Song, Tongyu Liu, Junfeng Qi, Longping Zhang   +8 more
doaj   +1 more source

Research on the estimation method of the point-of-interest (POI) displacement for ultra-precision flexible motion system based on functional optical fiber sensor

Mechanics & Industry, 2021
This paper proposes a POI displacement estimation method based on the functional optical fiber sensor and the phase modulation principle to improve the POI displacement estimation accuracy. First, the relation between the object deformation and the optic
Li Yujie, Zhang Ming, Zhu Yu
doaj   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells

FEBS Open Bio, EarlyView.
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley   +1 more source

MOGAD Is the Most Common Cause of Isolated Optic Neuritis in Children

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objectives The study aimed to characterize the clinical features, etiologies, and outcomes of isolated, first‐time pediatric ON in the post‐MOG‐IgG era. Methods This was a single‐center retrospective cohort study at Texas Children's Hospital of patients diagnosed with first‐time ON between 2018–2024, with follow‐up data collected through 2025.
Chaitanya Aduru, Akansha Chandrasekar, Kyla Blasingame, Jonathan Rosen, Jesse M. Levine, Karla Salazar, Madhuri Chilakapati, Rod Foroozan, Victoria Hardwick, Jonathan M. Yarimi, Nikita Shukla, Timothy E. Lotze, Kristen S. Fisher, Alexander J. Sandweiss   +13 more
wiley   +1 more source

A Large Area Fiber Optic Gyroscope on multiplexed fiber network [PDF]

, 2013
We describe a fiber optical gyroscope based on the Sagnac effect realized on a multiplexed telecom fiber network. Our loop encloses an area of 20 km2 and coexists with Internet data traffic.
LEVI, FILIPPO, G. A. Costanzo, Levi, F., Costanzo, Giovanni Antonio, A. Mura, Pizzocaro, Marco, CALONICO, DAVIDE, M. Pizzocaro, D. Calonico, Clivati, Cecilia, Calonico, D., C. Clivati, F. Levi, Mura, A.   +13 more
core   +1 more source