Results 121 to 130 of about 584,548 (310)

Low-Coherence Interferometric Imaging: Solution of the One-Dimensional Inverse Scattering Problem [PDF]

open access: yes, 2004
Optical coherence tomography (OCT) is a non-invasive imaging technique based on the use of light sources exhibiting a low degree of coherence. Low coherence interferometric microscopes have been successful in producing internal images of thin pieces of ...
Chaubell, Mario Julián
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Exploiting multimode waveguides for pure fibre-based imaging

open access: yes, 2012
We acknowledge support from the UK Engineering and Physical Science Research CouncilThere has been an immense drive in modern microscopy towards miniaturisation and fibre based technology.
Tomáš Čižmár   +3 more
core   +1 more source

Imaging fibre bundles for Fizeau-based optical coherence tomography [PDF]

open access: yes, 2008
An OCT system incorporating a coherent fibre imaging bundle is described. Fibres are accessed sequentially by a beam focused onto the input face of the bundle, allowing 2D or 3D images to be acquired using point detection.
Tatam, Ralph P.   +2 more
core   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Investigation of a fibre-optic Fizeau interferometer configuration and coherent fibre-optic imaging bundles for optical coherence tomography [PDF]

open access: yes, 2008
Currently several medical imaging techniques are in operation in clinical environments that help the doctors to diagnose diseases before proceeding to treatment. This research investigated and implemented configurations for a medical imaging technique
Sarantavgas, Georgios
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Tailoring optical fibers for cell transfection

open access: yes, 2012
Optical transfection is a promising technique for the delivery of foreign genetic material into cells by transiently changing the permeability of the cell membrane.
Ma, Nan
core  

Remote sensing of strong emotions using electro-optical imaging technique [PDF]

open access: yes, 2013
©Cranfield UniversityThis thesis reports a summary of the PhD programme for the assessment of person‘s emotional anxiety using Electro-optical technology. The thesis focuses mainly on the understanding of fundamental properties of physiological responses
Hong, Kan
core  

Intensity interferometry : Optical imaging with kilometer baselines

open access: yes, 2016
Optical imaging with microarcsecond resolution will reveal details across and outside stellar surfaces but requires kilometer-scale interferometers, challenging to realize either on the ground or in space.
Dravins, Dainis   +2 more
core   +1 more source

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