Results 111 to 120 of about 216,176 (317)

Fiber-optic Endomicroscopy for Label-free Optical Histology

open access: yesCLEO: 2015, 2015
We report on recently developed high-resolution, fiber-optic scanning endomicroscopy technology and its capability of performing label-free optical histologic imaging in vivo and in situ. Technological advances and examples of biological tissue imaging will be presented.
openaire   +2 more sources

Matrigel inhibits elongation and drives endoderm differentiation in aggregates of mouse embryonic stem cells

open access: yesFEBS Open Bio, EarlyView.
Stem cell‐based embryo models (SCBEMs) are valuable to study early developmental milestones. Matrigel, a basement membrane matrix, is a critical substrate used in various SCBEM protocols, but its role in driving stem cell lineage commitment is not clearly defined.
Atoosa Amel   +3 more
wiley   +1 more source

Iron‐dependent lysosomal LDL oxidation induces the expression of scavenger receptor A in human THP‐1 monocytes

open access: yesFEBS Open Bio, EarlyView.
In human monocytic cells THP‐1, a limited uptake of native—not oxidized—LDL/VLDL induced expression of scavenger receptor A and cellular adhesion. Induction was inhibited by lysosomotropic (WR‐1065) and lipophilic (BHT) antioxidants and by siRNAs against ferritinophagy.
Martina Čierna   +4 more
wiley   +1 more source

An approach for coherent periodogram averaging of tilt‐series data for improved contrast transfer function estimation

open access: yesFEBS Open Bio, EarlyView.
The contrast transfer function (CTF) is an imaging aberration that is a major resolution‐limiting factor in cryo‐electron microscopy (cryo‐EM). Precise CTF estimation is key to overcoming this limitation, but is particularly challenging in cryo‐electron tomography (cryo‐ET) data. Here, we present an approach for using geometric information to assist in
Sagar Khavnekar, William Wan
wiley   +1 more source

Feasibility of 3D Reconstruction of Neural Morphology Using Expansion Microscopy and Barcode-Guided Agglomeration

open access: yesFrontiers in Computational Neuroscience, 2017
We here introduce and study the properties, via computer simulation, of a candidate automated approach to algorithmic reconstruction of dense neural morphology, based on simulated data of the kind that would be obtained via two emerging molecular ...
Young-Gyu Yoon   +12 more
doaj   +1 more source

Label-free optical imaging of membrane potential

open access: yesCurrent Opinion in Biomedical Engineering, 2019
Offering high temporal resolution, voltage imaging is an important and essential technique in neuroscience. Among different optical imaging approaches, the label-free approach remains attractive due to its unique value coming from free of exogenous chromophores.
Ji-Xin Cheng   +3 more
openaire   +3 more sources

Knockout of the mitoribosome rescue factors Ict1 or Mtrfr is viable in zebrafish but not mice: compensatory mechanisms underlying each factor's loss

open access: yesFEBS Open Bio, EarlyView.
Mitochondria contain two mitoribosome rescue factors, ICT1 and MTRFR (C12orf65). ICT1 also functions as a mitoribosomal protein in mice and humans, and its loss is lethal. Although Mtrfr knockout mice could not be generated, knockout zebrafish lines for ict1 and mtrfr were established.
Nobukazu Nameki   +11 more
wiley   +1 more source

Profiling the effect of low frequency mechanical vibration on the metabolic and oxidative stress responses of A431 carcinoma

open access: yesFEBS Open Bio, EarlyView.
20 Hz mechanical vibration induced A431 cancer cells' apoptosis without such effect on other healthy cell lines of L929 and C2C12. Lowered glucose consumption is observed specifically in A431. The expressions of ROS, HMGB1, and HSP1 levels initially increase and subsequently decrease in the cancer cell line, as opposed to L929 and C2C12, which ...
Wresti L. Anggayasti   +5 more
wiley   +1 more source

Humans in the loop: Community science and machine learning synergies for overcoming herbarium digitization bottlenecks

open access: yesApplications in Plant Sciences
Premise Among the slowest steps in the digitization of natural history collections is converting imaged labels into digital text. We present here a working solution to overcome this long‐recognized efficiency bottleneck that leverages synergies between ...
Robert Guralnick   +11 more
doaj   +1 more source

The use of Optical Character Recognition (OCR) in the digitisation of herbarium specimen labels

open access: yesPhytoKeys, 2014
At the Royal Botanic Garden Edinburgh (RBGE) the use of Optical Character Recognition (OCR) to aid the digitisation process has been investigated. This was tested using a herbarium specimen digitisation process with two stages of data entry. Records were
Robyn Drinkwater   +2 more
doaj   +1 more source

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