Results 71 to 80 of about 341,270 (292)
FEBS Open Bio, EarlyView.RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
FEBS Open Bio, EarlyView.CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
Nonlinear optical switching matrix
Optics Letters, 2003 We show that nonlinear directional couplers with variable coupling coefficients can be used to compose a special kind of switching matrix. The switching matrix can be controlled by the signal itself or by a strong pump. Light routing is realized by adjustment of the power of the signal or the pump.
Gang Jun, Liu +4 more
openaire +2 more sources
Technology development and evolution trend of all-optical switching networks
Dianxin kexue, 2019 In the 5G era,growth of network traffic is driven by the development of emerging services,especially the burst of east-west traffic.The network evolved from a multi-layered planar architecture to a three-dimensional architecture,thereby promoting the ...
Min ZHOU, Jian ZHANG, Yin WANG
doaj +2 more sources
WDM-compatible multimode optical switching system-on-chip
Nanophotonics, 2019 The development of optical interconnect techniques greatly expands the communication bandwidth and decreases the power consumption at the same time. It provides a prospective solution for both intra-chip and inter-chip links.
Jia Hao +6 more
doaj +1 more source
FEBS Open Bio, EarlyView.Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
Resource Reservation Schemes for Optical Burst Switching Networks
Communications, 2016 Since the current optical communication networks are based on internet protocol and demands for transmission capacity and speed are growing, the optical burst switching presents the solution for future high-speed wavelength division multiplexing optical ...
Michaela Solanska +2 more
doaj +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
All-optical switching with transverse optical patterns [PDF]
Physical Review A, 2008 We demonstrate an all-optical switch that operates at ultra-low-light levels and exhibits several features necessary for use in optical switching networks. An input switching beam, wavelength $ $, with an energy density of $10^{-2}$ photons per optical cross section [$ = ^2/(2 )$] changes the orientation of a two-spot pattern generated via ...
Dawes, Andrew M.C. +3 more
openaire +2 more sources