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Introduction to Optical Tweezers
2016Thirty years after their invention by Arthur Ashkin and colleagues at Bell Labs in 1986 [1], optical tweezers (or traps) have become a versatile tool to address numerous biological problems. Put simply, an optical trap is a highly focused laser beam that is capable of holding and applying forces to micron-sized dielectric objects.
Matthias D. Koch, Joshua W. Shaevitz
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Optical tweezers in cell biology [PDF]
Trends in Cell Biology, 1992 The authors are at the Department of Cell Biology, Box 3709, Duke University Medical Center, Durham, NC 27710, USA. The invention of the single-beam optical gradient trap I-3, known less formally as optical tweezers, has opened possibilities in cell biology that are only beginning to be re- alized.
Michael P. Sheetz, Scot C. Kuo
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Advantages of Holographic Optical Tweezers
Novel Optical Instrumentation for Biomedical Applications, 2003In the last decade optical tweezers became an important tool in microbiology. However the setup becomes very complex if more than one trap needs to be moved. Holographic tweezers offer a very simple and cost efficient way of manipulating several traps independently in all three dimensions with an accuracy of less than 100 nm.
Tobias Haist +3 more
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Optical Tweezers and Optofluidics
2006 Digest of the LEOS Summer Topical Meetings, 2006In this paper, the authors demonstrated the use of optically trapped microspheres to tune the transmission properties of embedded fiber optic waveguides, and the use of optical tweezers for optical actuation of cantilevers for use as switches and sensors.
Mark Cronin-Golomb +2 more
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Optical tweezers stretching of chromatin
2003Recently significant success has emerged from exciting research involving chromatin stretching using optical tweezers. These experiments, in which a single chromatin fibre is attached by one end to a micron-sized bead held in an optical trap and to a solid surface or second bead via the other end, allows manipulation and force detection at a single ...
Lisa H. Pope +2 more
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Is it possible to enlarge the trapping range of optical tweezers via a single beam?
Applied Physics Letters, 2019For optical tweezers, a tiny focal spot of the trapping beam is necessary for providing sufficient intensity-gradient force. This condition results in a limited small trapping range to guarantee stable trapping of the particle.
X. Z. Li +6 more
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2010
The technical development of optical tweezers, along with their application in the biological and physical sciences, has progressed significantly since the demonstration of an optical trap for micron-sized particles based on a single, tightly focused laser beam was first reported more than twenty years ago.
David McGloin +2 more
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The technical development of optical tweezers, along with their application in the biological and physical sciences, has progressed significantly since the demonstration of an optical trap for micron-sized particles based on a single, tightly focused laser beam was first reported more than twenty years ago.
David McGloin +2 more
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2017
This book gives an accessible, detailed overview on techniques of single molecule biophysics (SMB), showing how they are applied to numerous biological problems associated with understanding the molecular mechanisms of DNA replication, transcription, and translation, as well as functioning of molecular machines.
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This book gives an accessible, detailed overview on techniques of single molecule biophysics (SMB), showing how they are applied to numerous biological problems associated with understanding the molecular mechanisms of DNA replication, transcription, and translation, as well as functioning of molecular machines.
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Potential mapping of optical tweezers
Optics Letters, 2011Optical tweezers are very often used for measurement of piconewton range forces. Depending on the displacement of the trapped bead, the trap may become stiffer which causes considerable underestimation of the measured force. We have shown, both by theory and experiment, that such a stiffening occurs for beads larger than 0.5 μm in radius. For the first
Rouzbeh Shokri +2 more
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Technical Digest. Summaries of papers presented at the Conference on Lasers and Electro-Optics. Postconference Technical Digest (IEEE Cat. No.01CH37170), 2001
Summary form only given. A compact optical tweezer package has been developed for use on a microscope to be flown on the International Space Station as part of a series of experiments in colloid crystalization. A brief introduction to the principles of single-beam optical tweezer operation is presented, after which a detailed system layout is shown ...
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Summary form only given. A compact optical tweezer package has been developed for use on a microscope to be flown on the International Space Station as part of a series of experiments in colloid crystalization. A brief introduction to the principles of single-beam optical tweezer operation is presented, after which a detailed system layout is shown ...
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